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Rac1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-82169

Novus Biologicals, part of Bio-Techne
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NB100-82169
NB100-82169-0.025ml

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The antiserum was produced against synthesized non-phosphopeptide derived from human Rac1 around amino acids 69~73 (P-L-S-Y-P).

Specificity

Detects endogenous levels of total Rac1 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Rac1 Antibody

Immunocytochemistry/ Immunofluorescence: Rac1 Antibody [NB100-82169]

Immunocytochemistry/ Immunofluorescence: Rac1 Antibody [NB100-82169]

Immunocytochemistry/Immunofluorescence: Rac1 Antibody [NB100-82169] - Staining of methanol-fixed Hela cells using Rac1 (Ab-71) antibody
Rac1 Antibody

Western Blot: Rac1 Antibody [NB100-82169] -

Western Blot: Rac1 Antibody [NB100-82169] - Western blot analysis of extracts from various cells using NB100-82169

Applications for Rac1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:200

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rac1

Rac1 encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene. Aoki K, et al. (2005) Mol Biol Cell; 16(5): 2207

Long Name

Ras-related C3 Botulinum Toxin Substrate 1 (Rho Family, small GTP binding Protein Rac1)

Alternate Names

p21-Rac1, TC-25

Entrez Gene IDs

19353 (Mouse); 363875 (Rat)

Gene Symbol

RAC1

UniProt

Additional Rac1 Products

Product Documents for Rac1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rac1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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