Rac1 [p Ser71] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-38637
Key Product Details
Species Reactivity
Human, Rat
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic phosphorylated peptide around S71 of human Rac1 (NP_008839.2).
Sequence:
PLSYP
Sequence:
PLSYP
Modification
p Ser 71
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Rac1 [p Ser71] Antibody
![Rac1 [p Ser71] Antibody](https://resources.bio-techne.com/images/products/nbp3-38637_rabbit-rac1-p-ser-71-pab-912202414123882.jpg "Western Blot: Rac1 [p Ser71] Antibody [NBP3-38637] -")
Western Blot: Rac1 [p Ser71] Antibody [NBP3-38637] -
Western Blot: Rac1 [p Ser71] Antibody [NBP3-38637] - Western blot analysis of lysates from A-431 cells, using Rac1 Rabbit pAb at 1:1000 dilution. A-431 cells were treated by EGF (25 ug/mL) at 37C for 30 minutes after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s.
Applications for Rac1 [p Ser71] Antibody
Application
Recommended Usage
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Rac1
Long Name
Ras-related C3 Botulinum Toxin Substrate 1 (Rho Family, small GTP binding Protein Rac1)
Alternate Names
p21-Rac1, TC-25
Gene Symbol
RAC1
Additional Rac1 Products
Product Documents for Rac1 [p Ser71] Antibody
Product Specific Notices for Rac1 [p Ser71] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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