RAC2 Antibody (3B8)

Novus Biologicals, part of Bio-Techne | Catalog # H00005880-M08

Novus Biologicals, part of Bio-Techne
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H00005880-M08
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3B8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RAC2 Antibodies »

Product Specifications

Immunogen

RAC2 (AAH01485.1, 1 a.a. ~ 192 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDSKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASYENVRAKWFPEVRHHCPSTPIILVGTKLDLRDDKDTIEKLKEKKLAPITYPQGLALAKEIDSVKYLECSALTQRGLKTVFDEAIRAVLCPQPTRQQKRACSLL

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for RAC2 Antibody (3B8)

Western Blot: RAC2 Antibody (3B8) [H00005880-M08]

Western Blot: RAC2 Antibody (3B8) [H00005880-M08]

Western Blot: RAC2 Antibody (3B8) [H00005880-M08] - Analysis of RAC2 expression in transfected 293T cell line by RAC2 monoclonal antibody (M08), clone 3B8. Lane 1: RAC2 transfected lysatE (21.4 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: RAC2 Antibody (3B8) [H00005880-M08]

Immunocytochemistry/ Immunofluorescence: RAC2 Antibody (3B8) [H00005880-M08]

Immunocytochemistry/Immunofluorescence: RAC2 Antibody (3B8) [H00005880-M08] - Analysis of monoclonal antibody to RAC2 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: RAC2 Antibody (3B8) [H00005880-M08] - Detection limit for recombinant GST tagged RAC2 is 1 ng/ml as a capture antibody.

Applications for RAC2 Antibody (3B8)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for ELISA and WB.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RAC2

RAC2 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, RAC2 binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. There are two isoforms of RAC2; isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.

Alternate Names

GX, HSPC022, ras-related C3 botulinum toxin substrate 2 (rho family, small GTP bindingprotein Rac2), rho family, small GTP binding protein Rac2, small GTP-bindingprotein Rac2)

Gene Symbol

RAC2

UniProt

AAH01485 (Human)

Additional RAC2 Products

Product Documents for RAC2 Antibody (3B8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RAC2 Antibody (3B8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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