RAC2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31086

Novus Biologicals, part of Bio-Techne
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NBP2-31086
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all RAC2 Antibodies »

Product Summary for RAC2 Antibody

Immunogen

Peptide with sequence PQPTRQQKRACSLL, from the C Terminus of the protein sequence according to NP_002863.1.

Reactivity Notes

Expected reactivity: Mouse, Rat

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for RAC2 Antibody

Western Blot: RAC2 Antibody [NBP2-31086]

Western Blot: RAC2 Antibody [NBP2-31086]

Western Blot: RAC2 Antibody [NBP2-31086] - (0.1ug/ml) staining of Molt4 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: RAC2 Antibody [NBP2-31086]

Immunohistochemistry-Paraffin: RAC2 Antibody [NBP2-31086]

Immunohistochemistry-Paraffin: RAC2 Antibody [NBP2-31086] - (2ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Applications for RAC2 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

2-4 ug/ml

Peptide ELISA

1:32000

Western Blot

0.1-3 ug/ml
Application Notes
Western Blot: Approx 22kDa band observed in lysates of Human T cell line MOLT4 (calculated MW of 21.4kDa according to NP_002863.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RAC2

RAC2 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, RAC2 binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. There are two isoforms of RAC2; isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.

Alternate Names

GX, HSPC022, ras-related C3 botulinum toxin substrate 2 (rho family, small GTP bindingprotein Rac2), rho family, small GTP binding protein Rac2, small GTP-bindingprotein Rac2)

Gene Symbol

RAC2

Additional RAC2 Products

Product Documents for RAC2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RAC2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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