Rad17 [p Ser656] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13072

Novus Biologicals, part of Bio-Techne
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NBP3-13072
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Rad17 Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser656 of human Rad17. The exact sequence is proprietary.

Modification

p Ser 656

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Rad17 [p Ser656] Antibody

Western Blot: Rad17 [p Ser656] Antibody [NBP3-13072]

Western Blot: Rad17 [p Ser656] Antibody [NBP3-13072]

Western Blot: Rad17 [p Ser656] Antibody [NBP3-13072] - Untreated (-) and treated (+) 293T whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rad17 (phospho Ser656) antibody (NBP3-13072) diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: Rad17 [p Ser656] Antibody [NBP3-13072]

Immunocytochemistry/ Immunofluorescence: Rad17 [p Ser656] Antibody [NBP3-13072]

Immunocytochemistry/Immunofluorescence: Rad17 [p Ser656] Antibody [NBP3-13072] - Rad17 (phospho Ser656) antibody detects Rad17 (phospho Ser656) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Rad17 (phospho Ser656) protein stained by Rad17 (phospho Ser656) antibody (NBP3-13072) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.
Immunohistochemistry-Paraffin: Rad17 [p Ser656] Antibody [NBP3-13072]

Immunohistochemistry-Paraffin: Rad17 [p Ser656] Antibody [NBP3-13072]

Immunohistochemistry-Paraffin: Rad17 [p Ser656] Antibody [NBP3-13072] - Rad17 (phospho Ser656) antibody detects Rad17 (phospho Ser656) protein at nucleus in human oral carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. Rad17 (phospho Ser656) antibody (NBP3-13072) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Applications for Rad17 [p Ser656] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rad17

Signals originating from damaged DNA activate checkpoint pathways and target the mitotic apparatus via a number a distinct mechanisms. These checkpoints link damage to DNA with the components of the cell cycle through signal transduction systems. In mammals, five genes have been identified as components of DNA damage-induced checkpoint pathways: ATM, p53, p21/WAF1/CIP1, hCHK1, and 14-3-3sigma. When overexpressed in mammalian cells, hRAD17 activates p53 and causes an accumulation of G1 phase cells, suggesting an involvement of the hRAD17 gene in this checkpoint pathway.(1).

Alternate Names

CCYC, cell cycle checkpoint protein (RAD17), cell cycle checkpoint protein RAD17, FLJ41520, hRad17, R24L, RAD1 (S. pombe) homolog, RAD1 homolog, RAD17 homolog (S. pombe), Rad17-like protein, RAD17Sp, Rad24, RF-C activator 1 homolog, RF-C/activator 1 homolog

Gene Symbol

RAD17

Additional Rad17 Products

Product Documents for Rad17 [p Ser656] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rad17 [p Ser656] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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