Rad23 Antibody (3B7T9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16573

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16573-100ul
NBP3-16573-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3B7T9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Rad23 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Rad23 (P54725). TGIPGSPEPEHGSVQESQVSEQPATEAAGENPLEFLRDQPQFQNMRQVIQQNPALLPALLQQLGQENPQLLQQISRHQEQFIQMLNEPPGELADISDVEGE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rad23 Antibody (3B7T9)

Western Blot: Rad23 Antibody (3B7T9) [NBP3-16573]

Western Blot: Rad23 Antibody (3B7T9) [NBP3-16573]

Western Blot: Rad23 Antibody (3B7T9) [NBP3-16573] - Western blot analysis of extracts of various cell lines, using Rad23 Rabbit mAb (NBP3-16573) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.
Immunocytochemistry/ Immunofluorescence: Rad23 Antibody (3B7T9) [NBP3-16573]

Immunocytochemistry/ Immunofluorescence: Rad23 Antibody (3B7T9) [NBP3-16573]

Immunocytochemistry/Immunofluorescence: Rad23 Antibody (3B7T9) [NBP3-16573] - Immunofluorescence analysis of U-2 OS cells using Rad23 Rabbit mAb (NBP3-16573) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: Rad23 Antibody (3B7T9) [NBP3-16573]

Western Blot: Rad23 Antibody (3B7T9) [NBP3-16573]

Western Blot: Rad23 Antibody (3B7T9) [NBP3-16573] - Western blot analysis of extracts of various cell lines, using Rad23 Rabbit mAb (NBP3-16573) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
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Applications for Rad23 Antibody (3B7T9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Rad23

Rad23 is an evolutionarily conserved protein that is important for nucleotide excision repair. All eukaryotic Rad23 proteins contain an amino-terminal UbL domain that bears a striking resemblance to ubiquitin. Rad23 has a proteolytic function in DNA repair via its interaction with the 26S proteosome through the UbL domain. The elimination of this Rad23 domain causes sensitivity to ultraviolet radiation.

Alternate Names

hHR23

Gene Symbol

RAD23A

Additional Rad23 Products

Product Documents for Rad23 Antibody (3B7T9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Rad23 Antibody (3B7T9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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