Rad23 Antibody (3C12)

Novus Biologicals, part of Bio-Techne | Catalog # H00005886-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Knockdown Validated, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3C12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Rad23 Antibodies »

Product Specifications

Immunogen

RAD23A (AAH14026, 151 a.a. ~ 250 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EDAASTLVTGSEYETMLTEIMSMGYERERVVAALRASYNNPHRAVEYLLTGIPGSPEPEHGSVQESQVSEQPATEAAGENPLEFLRDQPQFQNMRQVIQQ

Specificity

RAD23A - RAD23 homolog A (S. cerevisiae)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Rad23 Antibody (3C12)

Western Blot: Rad23 Antibody (3C12) [H00005886-M01]

Western Blot: Rad23 Antibody (3C12) [H00005886-M01]

Western Blot: Rad23 Antibody (3C12) [H00005886-M01] - Analysis of RAD23A over-expressed 293 cell line, cotransfected with RAD23A Validated Chimera RNAi ( Cat # H00005886-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RAD23A monoclonal antibody (M01), clone 3C12 (Cat # H00005886-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: Rad23 Antibody (3C12) [H00005886-M01]

Western Blot: Rad23 Antibody (3C12) [H00005886-M01]

Western Blot: Rad23 Antibody (3C12) [H00005886-M01] - Analysis of RAD23A expression in transfected 293T cell line by RAD23A monoclonal antibody (M01), clone 3C12. Lane 1: RAD23A transfected lysate (39.6 KDa). Lane 2: Non-transfected lysate.
Sandwich ELISA: Rad23 Antibody (3C12) [H00005886-M01] - Detection limit for recombinant GST tagged RAD23A is approximately 0.03ng/ml as a capture antibody.

Applications for Rad23 Antibody (3C12)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Rad23

The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.

Alternate Names

hHR23

Entrez Gene IDs

5886 (Human)

Gene Symbol

RAD23A

OMIM

600061 (Human)

UniProt

AAH14026 (Human)

Additional Rad23 Products

Product Documents for Rad23 Antibody (3C12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Rad23 Antibody (3C12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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