Rad51 Antibody (RAD51/2701)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79760

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-79760-100ug
NBP2-79760-20ug
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # RAD51/2701

Concentration

0.2 mg/ml

View all Rad51 Antibodies »

Product Summary for Rad51 Antibody (RAD51/2701)

Immunogen

Recombinant fragment of human Rad51 protein (around aa 1-134) (exact sequence is proprietary) (Uniprot: Q06609)

Localization

Nuclear

Marker

Prognostic and Response to Chemotherapy Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79895)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Rad51 Antibody (RAD51/2701)

Western Blot: Rad51 Antibody (RAD51/2701) [NBP2-79760]

Western Blot: Rad51 Antibody (RAD51/2701) [NBP2-79760]

Western Blot: Rad51 Antibody (RAD51/2701) [NBP2-79760] - Western Blot Analysis of HeLa, HepG2, K562, A431 and MCF-7 cell lysate using Rad51 Antibody (RAD51/2701).
Flow Cytometry: Rad51 Antibody (RAD51/2701) [NBP2-79760]

Flow Cytometry: Rad51 Antibody (RAD51/2701) [NBP2-79760]

Flow Cytometry: Rad51 Antibody (RAD51/2701) [NBP2-79760] - Flow Cytometric Analysis of K562 cells using Rad51 Antibody (RAD51/2701)followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Protein Array: Rad51 Antibody (RAD51/2701) [NBP2-79760]

Protein Array: Rad51 Antibody (RAD51/2701) [NBP2-79760]

Protein Array: Rad51 Antibody (RAD51/2701) [NBP2-79760] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Rad51 Antibody (RAD51/2701) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Applications for Rad51 Antibody (RAD51/2701)

Application
Recommended Usage

Flow Cytometry

1-2 ug/ml

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Rad51

Rad51 is involved in both the recombination and repair of DNA damage. Its function may be modulated by interaction with other repair proteins including BRCA1 and BRCA2. Alterations in the Rad51 gene have been associated with increased cancer risk. Rad51 is conserved across species and is homologous to prokaryotic RecA.

Alternate Names

BRCC5, hRAD51, HsRAD51, HsT16930, RAD51 (S. cerevisiae) homolog (E coli RecA homolog), RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae), RAD51 homolog A, RAD51ABRCA1/BRCA2-containing complex, subunit 5, RAD51L3, RecA, E. coli, homolog of, RECADNA repair protein RAD51 homolog 1, RecA-like protein, recombination protein A

Gene Symbol

RAD51

UniProt

Q06609

Additional Rad51 Products

Product Documents for Rad51 Antibody (RAD51/2701)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Rad51 Antibody (RAD51/2701)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.