RAD52 Antibody (7H5K0)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16584

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16584-100ul
NBP3-16584-20ul
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7H5K0

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RAD52 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 319-418 of human RAD52 (P43351). QELIKTLEDNSEKWAVTPDAGDGVVKPSSRADPAQTSDTLALNNQMVTQNRTPHSVCHQKPQAKSGSWDLQTYSADQRTTGNWESHRKSQDMKKRKYDPS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RAD52 Antibody (7H5K0)

Western Blot: RAD52 Antibody (7H5K0) [NBP3-16584]

Western Blot: RAD52 Antibody (7H5K0) [NBP3-16584]

Western Blot: RAD52 Antibody (7H5K0) [NBP3-16584] - Western blot analysis of extracts of various cell lines, using RAD52 Rabbit mAb (NBP3-16584) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Applications for RAD52 Antibody (7H5K0)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RAD52

DNA double-strand breaks are generated by ionizing radiation and endogenously produced radicals, and they often are repaired through the RAD52 homologous recombination pathway. The RAD52 family includes RAD51, RAD52, RAD54, RAD54B and MRE11 genes. Rad51 and Rad52 proteins perform the key steps in homologous recombination (HR), including the search for DNA homology and strand exchange, through similar mechanisms. Mre11 functions in both non-homologous end joining, and meiotic HR, and it is essential in mitosis for chromosome maintenance. Rad54 belongs to the SWI2/SNF2 subfamily of ATPases, which includes the DNA helicases involved in replication, recombination, and repair, as it contains seven amino acid sequence motifs that are largely conserved. Rad54 ATPase activity is dependent on double-stranded (ds) DNA, and the ATPase activity of Rad54 is not absolutely required for its DNA repair function, suggesting that these activities occur at distinct regions of the molecule. RAD54B is significantly homologous to the RAD54 recombination gene. Expression of RAD54B is highest in testis and spleen, which are active in both meiotic and mitotic recombination.

Alternate Names

DNA repair protein RAD52 homolog, RAD52 (S. cerevisiae) homolog, RAD52 homolog (S. cerevisiae), recombination protein RAD52, rhabdomyosarcoma antigen MU-RMS-40.23

Gene Symbol

RAD52

Additional RAD52 Products

Product Documents for RAD52 Antibody (7H5K0)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RAD52 Antibody (7H5K0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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