Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI7B11

Concentration

2 mg/ml

View all Rad9 Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human RAD9A expression vector

Specificity

This antibody is specific for Homo sapiens RAD9 homolog A (S. pombe) (RAD9A).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

42.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Rad9 Antibody (OTI7B11)

Western Blot: Rad9 Antibody (7B11) [NBP1-47946] Analysis of extracts (35ug) from 9 different cell lines by using anti-anti-Rad9monoclonal antibody.
Immunohistochemistry-Paraffin: Rad9 Antibody (OTI7B11) [NBP1-47946]

Immunohistochemistry-Paraffin: Rad9 Antibody (OTI7B11) [NBP1-47946]

Immunohistochemistry-Paraffin: Rad9 Antibody (7B11) [NBP1-47946] - Staining of paraffin-embedded colon tissue using anti-Rad9 mouse monoclonal antibody.
Western Blot: Rad9 Antibody (OTI7B11) [NBP1-47946]

Western Blot: Rad9 Antibody (OTI7B11) [NBP1-47946]

Western Blot: Rad9 Antibody (7B11) [NBP1-47946] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Rad9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Rad9.
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Applications for Rad9 Antibody (OTI7B11)

Application
Recommended Usage

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Rad9

This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity

Alternate Names

cell cycle checkpoint control protein RAD9A, DNA repair exonuclease rad9 homolog A, EC 3.1.11.2, hRAD9, RAD9, RAD9 (S. pombe) homolog, RAD9 homolog A (S. pombe)

Entrez Gene IDs

5883 (Human)

Gene Symbol

RAD9A

Additional Rad9 Products

Product Documents for Rad9 Antibody (OTI7B11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Rad9 Antibody (OTI7B11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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