Rad9 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87163

Novus Biologicals, part of Bio-Techne
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NBP1-87163
NBP1-87163-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Rad9 Antibodies »

Product Summary for Rad9 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AIFTIKDSLLDGHFVLATLSDTDSHSQDLGSPERHQPVPQLQAHSTPHPDDFANDDIDSYMIAMETTIGNEGSRVLPSISLSPGPQPPKSPGPHSEEEDEAEPSTVPGTPPPKKFRSLFFGSILAPV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rad9 Antibody

Western Blot: Rad9 Antibody [NBP1-87163]

Western Blot: Rad9 Antibody [NBP1-87163]

Western Blot: Rad9 Antibody [NBP1-87163] - Analysis in control (vector only transfected HEK293T lysate) and RAD9A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: Rad9 Antibody [NBP1-87163]

Immunocytochemistry/ Immunofluorescence: Rad9 Antibody [NBP1-87163]

Immunocytochemistry/Immunofluorescence: Rad9 Antibody [NBP1-87163] - Staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: Rad9 Antibody [NBP1-87163]

Immunohistochemistry-Paraffin: Rad9 Antibody [NBP1-87163]

Immunohistochemistry-Paraffin: Rad9 Antibody [NBP1-87163] - Staining in human small intestine and liver tissues using anti-RAD9A antibody. Corresponding RAD9A RNA-seq data are presented for the same tissues.
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Applications for Rad9 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rad9

Human Rad9 is a homologue of Schizosaccharomyces pombe Rad9 protein and plays a critical role in cell cycle checkpoint control.

Alternate Names

cell cycle checkpoint control protein RAD9A, DNA repair exonuclease rad9 homolog A, EC 3.1.11.2, hRAD9, RAD9, RAD9 (S. pombe) homolog, RAD9 homolog A (S. pombe)

Gene Symbol

RAD9A

Additional Rad9 Products

Product Documents for Rad9 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Rad9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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