Key Product Details

Species Reactivity

Mouse

Applications

Blockade of Receptor-ligand Interaction, CyTOF-ready, Flow Cytometry

Label

DyLight 680 (Excitation = 692 nm, Emission = 712 nm)

Antibody Source

Recombinant Monoclonal Rat IgG2A Clone # 199205

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Rae-1 Antibodies »

Product Specifications

Immunogen

BaF3 mouse pro-B cell line transfected with Rae-1 beta (Accession # NP_033043) and Mouse myeloma cell line NS0-derived recombinant mouse Rae-1 delta Leu29-Ser227 (Accession # Q9JI58)

Reactivity Notes

0

Specificity

Detects mouse Rae‑1 alpha/ beta/ delta/ epsilon. When tested in flow cytometry against a panel of transfectants expressing different Rae-1 proteins, this antibody strongly recognizes Rae-1 beta and Rae-1 delta. It shows weaker staining of Rae-1 alpha and Rae-1 epsilon transfectants. It does not recognize Rae-1 gamma.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Rae-1 Antibody (199205) [DyLight 680]

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

Optimal dilutions of this antibody should be experimentally determined.

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Rae-1

The Rae-1 proteins, including Rae-1 alpha, beta, gamma, delta, and epsilon, are members of a family of GPI-linked proteins that function as ligands for mouse NKG2D. The Rae-1 proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no ability to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are not found within the Major Histocompatibility Complex on mouse chromosome 17, but rather map to mouse chromosome 10. Rae-1 family members have retinoic acid-inducible elements in their promoter regions.

Long Name

Retinoic Acid Early Transcript 1

Alternate Names

Rae1

Gene Symbol

Raet1a

Additional Rae-1 Products

Product Documents for Rae-1 Antibody (199205) [DyLight 680]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Rae-1 Antibody (199205) [DyLight 680]

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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