Raf-1 [p Ser471] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23359

Novus Biologicals, part of Bio-Techne
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NBP3-23359
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Raf-1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Ser-471 in human Raf-1. This sequence has high homology with similar regions in rat and mouse Raf-1, and has high homology to the conserved sites in B-Raf (Ser-579) and A-Raf (Ser-432). [UniProt# P04049]

Modification

p Ser471

Specificity

This antibody was cross-adsorbed to phospho-Raf-1 (Ser-338) peptide before affinity purification using phospho-Raf-1 (Ser-471) peptide. The purified antibody detects a band at 74 kDa corresponding to C-Raf in western blots of human Jurkat cells treated with Calyculin A, but is not observed after lambda phosphatase treatment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for Raf-1 [p Ser471] Antibody

Raf-1 [p Ser471] Antibody

Western Blot: Raf-1 [p Ser471] Antibody [NBP3-23359] -

Western Blot: Raf-1 [p Ser471] Antibody [NBP3-23359] - Western blot of human Jurkat cells treated with calyculin A (100 nM) for 30 min. The blots were untreated (lanes 1 & 3) or treated (lanes 2 & 4) with lambda phosphatase and probed with anti-Raf-1 (N-terminal region) (lanes 1 & 2) or anti-Raf-1 (Ser-471) (lanes 3 & 4).

Applications for Raf-1 [p Ser471] Antibody

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Raf-1

Raf-1 is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. The activated Raf-1 can phosphorylate and activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the Serine/Threonine specific protein kinases ERK1 and ERK2. Downstream of Raf-1, activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation, and cell migration.

Long Name

v-raf-1Murine Leukemia Viral Oncogene Homolog 1

Alternate Names

c-Raf-1, Raf1, v-Raf-1

Gene Symbol

RAF1

Additional Raf-1 Products

Product Documents for Raf-1 [p Ser471] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Raf-1 [p Ser471] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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