RAG2 Antibody (2G8)

Novus Biologicals, part of Bio-Techne | Catalog # H00005897-M06

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Rat

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2G8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RAG2 Antibodies »

Product Specifications

Immunogen

RAG2 (NP_000527.1, 428 a.a. ~ 527 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NTWVPFYSTELNKPAMIYCSHGDGHWVHAQCMDLAERTLIHLSAGSNKYYCNEHVEIARALHTPQRVLPLKKPPMKSLRKKGSGKILTPAKKSFLRRLFD

Specificity

RAG2 - recombination activating gene 2 (2G8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for RAG2 Antibody (2G8)

Western Blot: RAG2 Antibody (2G8) [H00005897-M06]

Western Blot: RAG2 Antibody (2G8) [H00005897-M06]

Western Blot: RAG2 Antibody (2G8) [H00005897-M06] - Analysis of RAG2 expression in rat liver.
ELISA: RAG2 Antibody (2G8) [H00005897-M06]

ELISA: RAG2 Antibody (2G8) [H00005897-M06]

ELISA: RAG2 Antibody (2G8) [H00005897-M06] - Detection limit for recombinant GST tagged RAG2 is approximately 1ng/ml as a capture antibody.

Applications for RAG2 Antibody (2G8)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RAG2

This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms. [provided by RefSeq]

Alternate Names

RAG-2, recombination activating gene 2, V(D)J recombination-activating protein 2

Entrez Gene IDs

5897 (Human)

Gene Symbol

RAG2

UniProt

NP_000527 (Human)

Additional RAG2 Products

Product Documents for RAG2 Antibody (2G8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RAG2 Antibody (2G8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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