RalA/RalB Antibody (AT41E2) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-88840
![Western Blot: RalA/RalB Antibody (AT41E2)BSA Free [NBP2-88840]](https://resources.bio-techne.com/images/products/RalA-RalB-Antibody-AT41E2-Western-Blot-NBP2-88840-img0001.jpg "Western Blot: RalA/RalB Antibody (AT41E2)BSA Free [NBP2-88840]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # AT41E2
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant human RalA/RalB (1-203aa) purified from E. coli
Specificity
Detects both human RalA and RalB protein
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for RalA/RalB Antibody (AT41E2) - BSA Free
Western Blot: RalA/RalB Antibody (AT41E2)BSA Free [NBP2-88840]
Western Blot: RalA/RalB Antibody (AT41E2) [NBP2-88840] - The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RalA/RalB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Recombinant human RalA proteinLane 2.: Recombinant human RalB protein![Flow Cytometry: RalA/RalB Antibody (AT41E2) - BSA Free [NBP2-88840]](https://resources.bio-techne.com/images/products/RalA-RalB-Antibody-AT41E2-Flow-Cytometry-NBP2-88840-img0003.jpg "Flow Cytometry: RalA/RalB Antibody (AT41E2) - BSA Free [NBP2-88840]")
Flow Cytometry: RalA/RalB Antibody (AT41E2) - BSA Free [NBP2-88840]
Flow Cytometry: RalA/RalB Antibody (AT41E2) [NBP2-88840] - Flow cytometry analysis of RalA/RalB in HeLa cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
RalA/RalB Antibody (AT41E2) [NBP2-88840] - The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RalA/RalB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: HepG2 cell lysateLane 3.: NIH3T3 cell lysateLane 4.: PC3 cell lysateLane 5.: U87MG cell lysate
Applications for RalA/RalB Antibody (AT41E2) - BSA Free
Application
Recommended Usage
Flow Cytometry
2-5ug
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4), 10% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RalA/RalB
Long Name
Ras-like Protein A/B
Alternate Names
MGC48949, RAL, Ras family small GTP binding protein RALA, RAS-like protein A, ras-related protein Ral-A, v-ral simian leukemia viral oncogene homolog A (ras related)
Gene Symbol
RALA
Additional RalA/RalB Products
Product Documents for RalA/RalB Antibody (AT41E2) - BSA Free
Product Specific Notices for RalA/RalB Antibody (AT41E2) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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