RanGAP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33591

Novus Biologicals, part of Bio-Techne
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NBP2-33591
NBP2-33591-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RanGAP1 Antibodies »

Product Summary for RanGAP1 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: ETLAKTQVAGGQLSFKGKSLKLNTAEDAKDVIKEIEDFDSLEALRLEGNTVGVEAARVIAKALEKKSELKRCHWSDMFTGRLRTEIPPALISLGE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RanGAP1 Antibody

Western Blot: RanGAP1 Antibody [NBP2-33591]

Western Blot: RanGAP1 Antibody [NBP2-33591]

Western Blot: RanGAP1 Antibody [NBP2-33591] - Analysis using Anti-RANGAP1 antibody NBP2-33591 (A) shows similar pattern to independent antibody NBP2-56215 (B).
Immunocytochemistry/ Immunofluorescence: RanGAP1 Antibody [NBP2-33591]

Immunocytochemistry/ Immunofluorescence: RanGAP1 Antibody [NBP2-33591]

Immunocytochemistry/Immunofluorescence: RanGAP1 Antibody [NBP2-33591] - Staining of human cell line A-431 shows localization to nuclear membrane, cytosol & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RanGAP1 Antibody [NBP2-33591]

Immunohistochemistry-Paraffin: RanGAP1 Antibody [NBP2-33591]

Immunohistochemistry-Paraffin: RanGAP1 Antibody [NBP2-33591] - Staining in human testis and liver tissues using anti-RANGAP1 antibody. Corresponding RANGAP1 RNA-seq data are presented for the same tissues.
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Applications for RanGAP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RanGAP1

RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle.

Long Name

Ran GTPase Activating Protein 1

Alternate Names

Fug1

Gene Symbol

RANGAP1

UniProt

P46060 (Human)

Additional RanGAP1 Products

Product Documents for RanGAP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RanGAP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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