RANK/TNFRSF11A Antibody (9A725) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56508

Novus Biologicals, part of Bio-Techne
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NB100-56508
NB100-56508SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Avian

Cited:

Human, Mouse, Rat, Avian

Applications

Validated:

CyTOF-ready, Flow (Cell Surface), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow (Cell Surface), Flow Cytometry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 9A725

Format

BSA Free

Concentration

1.0 mg/ml

View all RANK/TNFRSF11A Antibodies »

Product Specifications

Immunogen

A fusion protein containing amino acid residues 326-616 of human RANK was used as immunogen. This antibody recognizes an epitope located between amino acid residues 330-427 of human RANK (NP_003830).

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 24376119). Avian reactivity reported in scientific literature (PMID: 31214852).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for RANK/TNFRSF11A Antibody (9A725) - BSA Free

Western Blot: RANK/TNFRSF11A Antibody (9A725)BSA Free [NB100-56508]

Western Blot: RANK/TNFRSF11A Antibody (9A725)BSA Free [NB100-56508]

Western Blot: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Analysis of RANK in transfected 293 cells using RANK antibody at 2 ug/ml.
Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) - BSA Free [NB100-56508]

Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) - BSA Free [NB100-56508]

Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) - BSA Free [NB100-56508] - An intracellular stain was performed on Raw264.7 cells with RANK/TNFRSF11A Antibody (9A725) NB100-56508 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).
Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) - BSA Free [NB100-56508]

Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) - BSA Free [NB100-56508]

Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Intracellular flow cytometry analysis of RANK in 10^6 RAW cells using 2 ug of RANK antibody. Shaded histogram represents RAW cells without antibody; green represents isotype control; antibody red represents RANK antibody.
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Applications for RANK/TNFRSF11A Antibody (9A725) - BSA Free

Application
Recommended Usage

Flow (Cell Surface)

reported in scientific literature (PMID 24376119)

Flow Cytometry

2-4 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1-2 ug/ml
Application Notes
Bharti et al (2004) published the RANK antibody clone 9A725 for Flow (Cell Surface). However, the epitope range recognized by this antibody is a potential cytoplasmic region as defined by www.uniprot.org. Hence, researchers are encouraged to refer to both the publication and the bioinformatics data bases for additional information in order to make their own determination regarding the suitability of the antibody for cell surface flow cytometry. In RAW cells, a 97 kDa is observed. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RANK/TNFRSF11A

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Receptor activator of NF-kB (RANK) is a recently cloned member of the TNFR superfamily with no significant homology to other members of this family. RANK ligand (RANKL/TRANCE/ OPGL) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival . The cytoplasmic domain of RANK interacts with TRAF2, TRAF5 and TRAF6. Overexpression of RANK activates NF-kB and c-Jun-terminal kinase (JNK) pathways. Recent studies have shown that RANK interaction with TRAF6 activates NF-kB, whereas JNK activation is mediated through binding of RANK to TRAF2.

Long Name

Receptor Activator of NF-kappa-B

Alternate Names

CD265, ODFR, TNFRSF11A, TRANCE R

Entrez Gene IDs

8792 (Human); 21934 (Mouse)

Gene Symbol

TNFRSF11A

UniProt

Q9Y6Q6

Additional RANK/TNFRSF11A Products

Product Documents for RANK/TNFRSF11A Antibody (9A725) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RANK/TNFRSF11A Antibody (9A725) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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