RAP1A Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-35089
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 39-138 of human RAP1A (NP_002875.1).
Sequence:
SYRKQVEVDCQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDERVVGKEQGQNLARQW
Sequence:
SYRKQVEVDCQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDERVVGKEQGQNLARQW
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for RAP1A Antibody
Immunohistochemistry: RAP1A Antibody [NBP3-35089] -
Immunohistochemistry: RAP1A Antibody [NBP3-35089] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using [KO Validated] RAP1A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry: RAP1A Antibody [NBP3-35089] -
Immunohistochemistry: RAP1A Antibody [NBP3-35089] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen using [KO Validated] RAP1A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Western Blot: RAP1A Antibody [NBP3-35089] -
Western Blot: RAP1A Antibody [NBP3-35089] - Western blot analysis of lysates from wild type(WT) and RAP1A knockdown (KD) 293T cells, using [KO Validated] RAP1A Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Applications for RAP1A Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:100
Immunohistochemistry-Paraffin
1:50 - 1:100
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Rap1A
Long Name
Ras-related Protein 1A
Alternate Names
KREV-1, SMGP21
Gene Symbol
RAP1A
Additional Rap1A Products
Product Documents for RAP1A Antibody
Product Specific Notices for RAP1A Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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