RAP74 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-17975

Novus Biologicals, part of Bio-Techne
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NBP3-17975
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RAP74 Antibodies »

Product Summary for RAP74 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human RAP74. The exact sequence is proprietary.

Reactivity Notes

Chimpanzee (97%), Rat (87%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RAP74 Antibody

Western Blot: RAP74 Antibody [NBP3-17975]

Western Blot: RAP74 Antibody [NBP3-17975]

Western Blot: RAP74 Antibody [NBP3-17975] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 7. 5% SDS PAGE gel, diluted at 1:3000.
Chromatin Immunoprecipitation (ChIP): RAP74 Antibody [NBP3-17975] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-TFIIF antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter or MyoD.
Western Blot: RAP74 Antibody [NBP3-17975]

Western Blot: RAP74 Antibody [NBP3-17975]

Western Blot: RAP74 Antibody [NBP3-17975] - Sample (30 ug of whole cell lysate) A: NIH-3T3 7. 5% SDS PAGE gel, diluted at 1:3000.
View 1 more images

Applications for RAP74 Antibody

Application
Recommended Usage

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RAP74

Enables several functions, including RNA polymerase II general transcription initiation factor activity; phosphatase activator activity; and promoter-specific chromatin binding activity. Involved in several processes, including positive regulation of transcription by RNA polymerase II; response to virus; and transcription initiation from RNA polymerase II promoter. Located in cell junction and nucleoplasm. Part of transcription factor TFIID complex.

Alternate Names

BTF4, General transcription factor IIF 74 kDa subunit, general transcription factor IIF subunit 1, general transcription factor IIF, polypeptide 1, 74kDa, TF2F1, TFIIF, TFIIF-alpha, Transcription initiation factor IIF subunit alpha, transcription initiation factor RAP74

Gene Symbol

GTF2F1

Additional RAP74 Products

Product Documents for RAP74 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RAP74 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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