Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2C3

Concentration

1 mg/ml

View all RAR alpha/NR1B1 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 52-299 of human RARA(NP_000955) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

50.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RAR alpha/NR1B1 Antibody (OTI2C3)

Western Blot: RAR alpha/NR1B1 Antibody (OTI2C3) [NBP2-45516]

Western Blot: RAR alpha/NR1B1 Antibody (OTI2C3) [NBP2-45516]

Western Blot: RAR alpha/NR1B1 Antibody (2C3) [NBP2-45516] - Analysis of HEK293T cells were transiently transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAR alpha.
Immunohistochemistry: RAR alpha/NR1B1 Antibody (OTI2C3) [NBP2-45516]

Immunohistochemistry: RAR alpha/NR1B1 Antibody (OTI2C3) [NBP2-45516]

Immunohistochemistry: RAR alpha/NR1B1 Antibody (2C3) [NBP2-45516] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120 C for 3min)
Immunohistochemistry: RAR alpha/NR1B1 Antibody (OTI2C3) [NBP2-45516]

Immunohistochemistry: RAR alpha/NR1B1 Antibody (OTI2C3) [NBP2-45516]

Immunohistochemistry: RAR alpha/NR1B1 Antibody (2C3) [NBP2-45516] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
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Applications for RAR alpha/NR1B1 Antibody (OTI2C3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Reported in scientific literature (PMID: 30560112)

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RAR alpha/NR1B1

Retinoic acid, a metabolite of vitamin A, is necessary for normal organogenesis but acts as a teratogen at high levels during embryonic and fetal development. Retinoic acid functions through its interaction with the nuclear protein, retinoic acid receptor (RAR). RAR belongs to the steroid and thyroid hormone superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. RAR exists as three major subtypes: alpha, beta and gamma.

Long Name

Retinoic Acid Receptor, alpha

Alternate Names

NR1B1, RARA

Gene Symbol

RARA

Additional RAR alpha/NR1B1 Products

Product Documents for RAR alpha/NR1B1 Antibody (OTI2C3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RAR alpha/NR1B1 Antibody (OTI2C3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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