Ras-GAP Antibody (2C12)

Novus Biologicals, part of Bio-Techne | Catalog # H00005921-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2C12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Ras-GAP Antibodies »

Product Specifications

Immunogen

RASA1 (NP_002881, 948 a.a. ~ 1047 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AKEPYMEGVNPFIKSNKHRMIMFLDELGNVPELPDTTEHSRTDLSRDLAALHEICVAHSDELRTLSNERGAQQHVLKKLLAITELLQQKQNQYTKTNDVR

Specificity

RASA1 - RAS p21 protein activator (GTPase activating protein) 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Ras-GAP Antibody (2C12)

Western Blot: Ras-GAP Antibody (2C12) [H00005921-M01]

Western Blot: Ras-GAP Antibody (2C12) [H00005921-M01]

Western Blot: Ras-GAP Antibody (2C12) [H00005921-M01] - RASA1 monoclonal antibody (M01), clone 2C12 Analysis of RASA1 expression in IMR-32.
Immunohistochemistry-Paraffin: Ras-GAP Antibody (2C12) [H00005921-M01]

Immunohistochemistry-Paraffin: Ras-GAP Antibody (2C12) [H00005921-M01]

Immunohistochemistry-Paraffin: Ras-GAP Antibody (2C12) [H00005921-M01] - Analysis of monoclonal antibody to RASA1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 6 ug/ml.
Sandwich ELISA: Ras-GAP Antibody (2C12) [H00005921-M01] - Detection limit for recombinant GST tagged RASA1 is 0.3 ng/ml as a capture antibody.

Applications for Ras-GAP Antibody (2C12)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Ras-GAP

The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues.

Long Name

GTPase activating protein

Alternate Names

CM-AVM, PKWS, RASA1, RasGAP

Entrez Gene IDs

5921 (Human)

Gene Symbol

RASA1

UniProt

NP_002881 (Human)

Additional Ras-GAP Products

Product Documents for Ras-GAP Antibody (2C12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ras-GAP Antibody (2C12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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