Ras-GAP Antibody (6W10G6)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33535
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 6W10G6
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Ras-GAP (P20936).
Sequence:
KSGSYLIRESDRRPGSFVLSFLSQMNVVNHFRIIAMCGDYYIGGRRFSSLSDLIGYYSHVSCLLKGEKLLYPVAPPEPVEDRRRVRAILPYTKVPDTDEIS
Sequence:
KSGSYLIRESDRRPGSFVLSFLSQMNVVNHFRIIAMCGDYYIGGRRFSSLSDLIGYYSHVSCLLKGEKLLYPVAPPEPVEDRRRVRAILPYTKVPDTDEIS
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
116 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Ras-GAP Antibody (6W10G6)
Western Blot: Ras-GAP Antibody (6W10G6) [NBP3-33535] -
Western Blot: Ras-GAP Antibody (6W10G6) [NBP3-33535] - Western blot analysis of various lysates using at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western Blot: Ras-GAP Antibody (6W10G6) [NBP3-33535] -
Western Blot: Ras-GAP Antibody (6W10G6) [NBP3-33535] - Western blot analysis of lysates from mouse brain, using at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.
Applications for Ras-GAP Antibody (6W10G6)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 ug/mL
Western Blot
1:100 - 1:500
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Ras-GAP
Long Name
GTPase activating protein
Alternate Names
CM-AVM, PKWS, RASA1, RasGAP
Gene Symbol
RASA1
Additional Ras-GAP Products
Product Documents for Ras-GAP Antibody (6W10G6)
Product Specific Notices for Ras-GAP Antibody (6W10G6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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