Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1H2

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Ras-GAP Antibodies »

Product Summary for Ras-GAP Antibody (OTI1H2) - Azide and BSA Free

Immunogen

Full length human recombinant rotein of human RASA1(NP_002881) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

116.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ras-GAP Antibody (OTI1H2) - Azide and BSA Free

Western Blot: Ras-GAP Antibody (OTI1H2)Azide and BSA Free [NBP2-73804]

Western Blot: Ras-GAP Antibody (OTI1H2)Azide and BSA Free [NBP2-73804]

Western Blot: Ras-GAP Antibody (OTI1H2) - Azide and BSA Free [NBP2-73804] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAS.
Immunocytochemistry/ Immunofluorescence: Ras-GAP Antibody (OTI1H2) - Azide and BSA Free [NBP2-73804]

Immunocytochemistry/ Immunofluorescence: Ras-GAP Antibody (OTI1H2) - Azide and BSA Free [NBP2-73804]

Immunocytochemistry/Immunofluorescence: Ras-GAP Antibody (OTI1H2) - Azide and BSA Free [NBP2-73804] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY RAS p21.

Applications for Ras-GAP Antibody (OTI1H2) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ras-GAP

Long Name

GTPase activating protein

Alternate Names

CM-AVM, PKWS, RASA1, RasGAP

Gene Symbol

RASA1

Additional Ras-GAP Products

Product Documents for Ras-GAP Antibody (OTI1H2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Ras-GAP Antibody (OTI1H2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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