RASGRF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13291

Novus Biologicals, part of Bio-Techne
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NBP3-13291
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RASGRF1 Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Rat RASGRF1. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for RASGRF1 Antibody

Western Blot: RASGRF1 Antibody [NBP3-13291]

Western Blot: RASGRF1 Antibody [NBP3-13291]

Western Blot: RASGRF1 Antibody [NBP3-13291] - Various tissue extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with RASGRF1 antibody (NBP3-13291) diluted at 1:1000 and competitor's antibody diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
RASGRF1 Antibody

Immunohistochemistry-Paraffin: RASGRF1 Antibody [NBP3-13291] -

Immunohistochemistry-Paraffin: RASGRF1 Antibody [NBP3-13291] - RASGRF1 antibody detects RASGRF1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
RASGRF1 stained by RASGRF1 antibody (NBP3-13291) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
RASGRF1 Antibody

Immunohistochemistry-Paraffin: RASGRF1 Antibody [NBP3-13291] -

Immunohistochemistry-Paraffin: RASGRF1 Antibody [NBP3-13291] - RASGRF1 antibody detects RASGRF1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
RASGRF1 stained by RASGRF1 antibody (NBP3-13291) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for RASGRF1 Antibody

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RASGRF1

RASGRF1 is a critical step in signal transduction responses to stimulation of cell surface receptors by their ligands involves the accumulation of Ras proteins in their active GTP-bound state. To reach their active GTP-bound state, Ras proteins must first release bound GDP, a rate limiting step mediated by a guanine nucleotide releasing factor (GRF). The mammalian Ras p21 GRF protein has been designated Ras-GRF1 p140. Ras-GRF1 accelerates release of GDP from H- and N-Ras p21 protein in vitro, but not from the related Ral A or Cdc42Hs GTP-binding proteins. Of interest, a region mapping within the amino terminal domain of Ras-GRF1 is similar to both the human breakpoint cluster protein, Bcr, and the Dbl proto-oncogene product, a guanine nucleotide-releasing factor for Cdc42Hs. Ras-GRF2 p135 has also been identified. Ras-GRF2 p135 is highly homologous to Ras-GRF1 p140 except in the region between the REM and CDC25 domains and appears to function similarly to Ras-GRF1 p140.

Alternate Names

CDC25guanine nucleotide exchange factor, CDC25L, GNRPguanine nucleotide-releasing factor 1, GRF1, GRF55, guanine nucleotide-releasing factor, 55 kD, Guanine nucleotide-releasing protein, H-GRF55, PP13187, Ras protein-specific guanine nucleotide-releasing factor 1, Ras-GRF1, ras-specific guanine nucleotide-releasing factor 1, Ras-specific guanine nucleotide-releasing factor, CDC25 homolog, Ras-specific nucleotide exchange factor CDC25

Gene Symbol

RASGRF1

Additional RASGRF1 Products

Product Documents for RASGRF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RASGRF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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