Rat BOK Antibody

R&D Systems, part of Bio-Techne | Catalog # AF825

R&D Systems, part of Bio-Techne
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AF825
AF825-SP
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Key Product Details

Species Reactivity

Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all BOK Antibodies »

Product Specifications

Immunogen

KLH-coupled rat BOK synthetic peptide
ARLLRAGLSWSAPERASPAPGC

Specificity

Detects rat BOK in Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rat BOK Antibody

Detection of Rat BOK antibody by Western Blot.

Detection of Rat BOK by Western Blot.

Western blot shows lysates ofE. colitransfected with an inducible expression vector containing rat BOK. PVDF membrane was probed with 0.8 µg/mL Rabbit Anti-Rat BOK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF825) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for BOK was detected at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Applications for Rat BOK Antibody

Application
Recommended Usage

Western Blot

0.8 µg/mL
Sample: E. coli transfected with an inducible expression vector containing rat BOK
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BOK

BOK (Bcl-2-related Ovarian Killer) is a 23-kDa pro-apoptotic member of the Bcl-2 family of proteins. BOK has three conserved Bcl-2 homology domains (BH1, 2, and 3) and a C-terminal transmembrane domain, but does not contain the anti-apoptotic BH4 domain. BOK interacts strongly with some anti-apoptotic Bcl-2 proteins (Mcl-1, BHRF1, and A1) and not at all with pro-apoptotic Bcl-2 proteins. The tissue distribution of BOK is restricted to reproductive tissues and it is highly expressed in ovary, testis, and uterus.

Long Name

Bcl-2-related Ovarian Killer

Alternate Names

bcl2-L-9, BCL2L9bcl-2-related ovarian killer protein, Bcl-2-like protein 9, BCL2-related ovarian killer, BOKL, hBOK, MGC4631

Entrez Gene IDs

666 (Human); 29884 (Rat)

Gene Symbol

BOK

Additional BOK Products

Product Documents for Rat BOK Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Rat BOK Antibody

For research use only

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