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Rat CD34 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF4117

R&D Systems, part of Bio-Techne
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BAF4117

Key Product Details

Species Reactivity

Rat

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat CD34
Glu37-Ile293
Accession # NP_001100672

Specificity

Detects rat CD34 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant porcine CD34 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Rat CD34 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Rat CD34
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD34

Rat CD34 is a 80-100 kDa type I transmembrane glycoprotein that belongs to the CD34/Podocalyxin family of sialomucins. It is a widely used marker of activated hematopoietic stem/progenitor cells and is also expressed on adult vascular endothelial cells. Mature rat CD34 is predicted to be a 352 amino acid (aa) protein with a 257 aa extracellular domain (ECD) (aa 35-291) and 74 aa cytoplasmic tail (aa 313-386). Over the region used as immunogen, rat CD34 shares 83% and 57% aa identity with mouse and human CD34, respectively.

Alternate Names

CD34, HPCA1

Entrez Gene IDs

947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)

Gene Symbol

CD34

UniProt

Additional CD34 Products

Product Documents for Rat CD34 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Rat CD34 Biotinylated Antibody

For research use only

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