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Rat CD4 Alexa Fluor® 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB65771G

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Rat

Applications

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # OX38

Product Specifications

Immunogen

MLR generated rat T cells

Specificity

Recognizes the rat CD4 cell surface antigen expressed by T helper cells, monocytes and macrophages. Clone OX38 competes for binding with clone W3/25.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Rat CD4 Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

Titration recommended for optimal concentration with starting range of 0.1-1 µg/1 million cells. Sample used for this experiment was Rat splenocytes.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD4

CD4 (cluster of differentiation 4), also known as L3T4 or T4, is a 55 kDa single chain type I transmembrane glycoprotein belonging to the immunoglobin (Ig) superfamily. CD4 is predominantly expressed on most thymocytes, a subset of mature T lymphocytes, and weakly on monocytes, tissue macrophages, dendritic cells, and granulocytes. It is also expressed on neurons and glial cells in the brain (1). CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. Either CD4 or CD8 expression is then lost giving rise to single positive (SP) CD4+ or CD8+ mature T cells. CD4+ SP cells (T helper cells) further differentiate into multiple subsets of CD4+ cells including Th1, Th2, Th17, Tfh, and Treg cells which regulate humoral and cellular immunity (2). The extracellular region of CD4 consists of 372 amino acids (aa) with four immunoglobin-like domains (D1-D4). The structures of D1 and D3 resemble variable (IgV) domains while D2 and D4 resemble constant (IgC) domains (3).

Given its critical role in T cell development, CD4 also has diverse immunology-related functions. CD4 acts as a coreceptor with the T-cell receptor (TCR) during T cell activation and thymic differentiation by binding directly to major histocompatibility complex (MHC) class II antigens and associating with the protein tyrosine kinase, Lck (4). This interaction contributes to the formation of the immunological synapse (5). Defects in antigen presentation cause dysfunction of CD4+ T cells and the almost complete loss of MHC II expression on B cells in peripheral blood, as observed in severe combined immunodeficiency (SCID) (6). CD4 also functions as a receptor for the human immunodeficiency virus (HIV) by binding to gp120, the envelope glycoprotein of HIV-1. It has been shown that the V-like domains are critical for binding to gp120 (7). In immune mediated and infectious diseases of the central nervous system, CD4 functions as an indirect mediator of neuronal damage (8).

References

  1. Omri, B. Crisanti, P. Alliot, F. Marty, M. Rutin, J. Levallois, C. Pessac, B. (1994). CD4 expression in neurons of the central nervous system. International Immunology, 6(3):377. doi:10.1093/intimm/6.3.377.
  2. Wan, Y.Y. & Flavell, R.A. (2009). How diverse-CD4 effector T cells and their functions. Journal of Molecular Cell Biology, 1(1):20-36. doi:10.1093/jmcb/mjp001.
  3. Wu, H. Myszka, D. G. Tendian, S.W. Brouillette, C.G. Sweet, R.W. Chaiken, I.M. & Hendrickson, W.A. (1996). Kinetic and structural analysis of mutant CD4 receptors that are defective in HIV gp120 binding. Proceedings of the National Academy of Sciences, 93(26):15030. doi:10.1073/pnas.93.26.15030.
  4. Doyle, C. & Strominger, J.L. (1987). Interaction between CD4 and class II MHC molecules mediates cell adhesion. Nature, 330:256. doi:10.1038/330256a0.
  5. Vignali, D.A. (2010). CD4 on the road to coreceptor status. The Journal of Immunology, 184(11):5933-5934. doi:10.4049/jimmunol.1090037.
  6. Tasher, D. & Dalal, I. (2012). The genetic basis of severe combined immunodeficiency and its variants. The Application of Clinical Genetics, 5:67-80. doi:10.2147/tacg.s18693.
  7. Arthos, J. Deen, K.C. Chaikin, M.A. Fornwald, J.A. Sathe, G. Sattentau, Q.J. Sweet, R.W. (1989). Identification of the residues in human CD4 critical for the binding of HIV. Cell, 57(3):469. doi:10.1016/0092-8674(89)90922-7.
  8. Buttini, M. Westland, C.E. Masliah, E. Yafeh, A.M. Wyss-Coray, T. Mucke, L. (1998). Novel role of human cd4 molecule identified in neurodegeneration. Nature Medicine, 4(4):441. doi:10.1038/nm0498-441.

Alternate Names

CD4

Entrez Gene IDs

920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)

Gene Symbol

CD4

Additional CD4 Products

Product Documents for Rat CD4 Alexa Fluor® 488-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Rat CD4 Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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