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Rat CD4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6439

R&D Systems, part of Bio-Techne
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MAB6439
MAB6439-SP

Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 743611

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat CD4
Lys28-Thr394
Accession # P05540

Specificity

Detects rat CD4 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant canine, cotton rat, feline, human, or mouse CD4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Rat CD4 Antibody

Detection of Rat CD4 antibody by Western Blot.

Detection of Rat CD4 by Western Blot.

Western blot shows lysates of rat spleen tissue and rat CD4+T Cells. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Rat CD4 Monoclonal Antibody (Catalog # MAB6439) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Rat CD4 Antibody

Application
Recommended Usage

Western Blot

0.2 µg/mL
Sample: Rat spleen tissue and rat CD4+ T Cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD4

CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations (1). Mature rat CD4 consists of a 367 amino acid (aa) extracellular region containing four immunoglobulin-like domains, a 23 aa transmembrane segment, and a 40 aa cytoplasmic domain (2). Within the ECD, rat CD4 shares 52% and 73% aa sequence identity with human and mouse CD4, respectively. CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. Either CD4 or CD8 expression is then lost, giving rise to single positive (SP) CD4+ or CD8+ mature T cells (3). CD4+ SP cells, also known as T helper cells, further differentiate into multiple subsets of CD4+ cells including Th1, Th2, Th17, Tfh, and Treg cells which regulate humoral and cellular immunity (4). CD4 is re‑expressed on circulating CD8+ T cells upon activation and contributes to their cytotoxic effector activity (5). In the rat, CD4 is additionally expressed on macrophages and selected subsets of dendritic cells (6, 7). Similar CD4 distribution between species cannot be assumed as demonstrated by its presence on macrophages in human and rat but not in mouse (6). CD4 binds directly to MHC class II molecules on antigen presenting cells (8). This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction (1, 9). Palmitoylation of two cysteine residues in the cytoplasmic tail of CD4 promotes the localization of CD4 in lipid rafts and its ability to augment TCR signaling via activation of the tyrosine kinase Lck (10). CD4 also functions as a chemotactic receptor for IL-16 and, in human, as a co‑receptor for the gp120 surface glycoprotein of HIV-1 (11‑14). CD4 associates with CD44 on the cell surface to potentiate CD44-mediated cell adhesion (15).

References

  1. Vignali, D.A.A. (2010) J. Immunol. 184:5933.
  2. Clark, S.J. et al. (1987) Proc. Natl. Acad. Sci. 84:1649.
  3. Alarcon, B. and H.M. van Santen (2010) Sci. Signal. 3:pe11.
  4. Wan, Y.Y. and R.A. Flavell (2009) Mol. Cell Biol. 1:20.
  5. Kitchen, S.G. et al. (2005) Proc. Natl. Acad. Sci. 102:3794.
  6. Crocker, P.R. et al. (1987) J. Exp. Med. 166:613.
  7. Liu, L. et al. (1998) J. Immunol. 161:1146.
  8. Doyle, C. and J.L. Strominger (1987) Nature 330:256.
  9. Huppa, J.B. et al. (2010) Nature 463:963.
  10. Fragoso, R. et al. (2003) J. Immunol. 170:913.
  11. Cruikshank, W.W. et al. (1994) Proc. Natl. Acad. Sci. 91:5109.
  12. Klatzmann, D. et al. (1984) Nature 312:767.
  13. Dagleish, A.G. et al. (1984) Nature 312:763.
  14. Biswas, P. et al. (2003) Blood 101:4452.
  15. Umberto D. et al. (1999) International Immunology 11:1085.

Alternate Names

CD4

Entrez Gene IDs

920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)

Gene Symbol

CD4

UniProt

Additional CD4 Products

Product Documents for Rat CD4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Rat CD4 Antibody

For research use only

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