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Rat CNTFR alpha Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF559

R&D Systems, part of Bio-Techne
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BAF559

Key Product Details

Species Reactivity

Rat

Applications

Immunohistochemistry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant rat CNTF R alpha
Ala19-Pro346
Accession # Q08406

Specificity

Detects rat CNTF R alpha in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rat CNTFR alpha Biotinylated Antibody

CNTF Ra antibody in Rat Brain by Immunohistochemistry (IHC-Fr).

CNTF R alpha in Rat Brain.

CNTF Ra was detected in perfusion fixed frozen sections of rat brain using 5 µg/mL Goat Anti-Rat CNTF Ra Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF559) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Rat CNTFR alpha Biotinylated Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat brain subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Western Blot

0.1 µg/mL
Sample: Recombinant Rat CNTF R alpha (Catalog # 558-CR)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CNTFR alpha

The high-affinity CNTF receptor complex, which mediates the biological action of CNTF, contains three proteins: the ligand-binding a subunit (CNTF R alpha) and the two signal-transducing proteins LIF R beta and gp130. Whereas LIF R beta and gp130 are widely expressed in many cell types, the expression of CNTF R alpha is restricted to the central and peripheral nervous systems. cDNAs encoding CNTF R alpha have been isolated from both human and rat and were shown to share 94% amino acid (aa) sequence identity. Rat CNTF R alpha cDNA encodes a 372 amino acid residue precursor protein that apparently has a 22 aa residue signal peptide and five potential glycoslyation sites. CNTF R alpha differs from other cytokine receptors in that it lacks transmembrane and cytoplasmic domains and is anchored to cell membranes by a glycosylphosphatidylinositol (GPI) linkage. Similar to other GPI-linked proteins, soluble CNTF receptor alpha (CNTF sR alpha) can be released from the cell surface by phosphatidylinositol-specific phospholipase C. CNTF sR alpha can be released from skeletal muscle in response to peripheral nerve injury and high concentrations of CNTF sR alpha have also been detected in human cerebrospinal fluid. CNTF sR alpha binds CNTF in solution and the complex can act on cells that express only LIF R beta and gp130 but not CNTF R alpha.

References

  1. Yancopoulos, G.D. in Guidebook to Cytokines and Their Receptors,
  2. Nicola, N.A. editor, Oxford University Press, New York, pp137.

Long Name

Ciliary Neurotrophic Factor Receptor alpha

Alternate Names

CNTF R alpha, CNTFR, CNTFRa

Entrez Gene IDs

1271 (Human); 12804 (Mouse); 313173 (Rat)

Gene Symbol

CNTFR

UniProt

Additional CNTFR alpha Products

Product Documents for Rat CNTFR alpha Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Rat CNTFR alpha Biotinylated Antibody

For research use only

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