Rat CNTFR alpha Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF559
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Ala19-Pro346
Accession # Q08406
Specificity
Clonality
Host
Isotype
Scientific Data Images for Rat CNTFR alpha Biotinylated Antibody
CNTF R alpha in Rat Brain.
CNTF Ra was detected in perfusion fixed frozen sections of rat brain using 5 µg/mL Goat Anti-Rat CNTF Ra Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF559) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Rat CNTFR alpha Biotinylated Antibody
Immunohistochemistry
Sample: Perfusion fixed frozen sections of rat brain subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Western Blot
Sample: Recombinant Rat CNTF R alpha (Catalog # 558-CR)
Formulation, Preparation, and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CNTFR alpha
The high-affinity CNTF receptor complex, which mediates the biological action of CNTF, contains three proteins: the ligand-binding a subunit (CNTF R alpha) and the two signal-transducing proteins LIF R beta and gp130. Whereas LIF R beta and gp130 are widely expressed in many cell types, the expression of CNTF R alpha is restricted to the central and peripheral nervous systems. cDNAs encoding CNTF R alpha have been isolated from both human and rat and were shown to share 94% amino acid (aa) sequence identity. Rat CNTF R alpha cDNA encodes a 372 amino acid residue precursor protein that apparently has a 22 aa residue signal peptide and five potential glycoslyation sites. CNTF R alpha differs from other cytokine receptors in that it lacks transmembrane and cytoplasmic domains and is anchored to cell membranes by a glycosylphosphatidylinositol (GPI) linkage. Similar to other GPI-linked proteins, soluble CNTF receptor alpha (CNTF sR alpha) can be released from the cell surface by phosphatidylinositol-specific phospholipase C. CNTF sR alpha can be released from skeletal muscle in response to peripheral nerve injury and high concentrations of CNTF sR alpha have also been detected in human cerebrospinal fluid. CNTF sR alpha binds CNTF in solution and the complex can act on cells that express only LIF R beta and gp130 but not CNTF R alpha.
References
- Yancopoulos, G.D. in Guidebook to Cytokines and Their Receptors, Nicola, N.A. editor, Oxford University Press, New York, pp137.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional CNTFR alpha Products
Product Documents for Rat CNTFR alpha Biotinylated Antibody
Product Specific Notices for Rat CNTFR alpha Biotinylated Antibody
For research use only