Rat CXCL2/GRO beta/MIP-2/CINC-3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB525
Key Product Details
Species Reactivity
Validated:
Rat
Cited:
Rat
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Neutralization, Western Blot
Cited:
ELISA Development
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 123802
Product Specifications
Immunogen
E. coli-derived recombinant rat CXCL2/GRO beta/MIP‑2/CINC‑3
Ser32-Asn100
Accession # P30348
Ser32-Asn100
Accession # P30348
Specificity
Detects rat CXCL2/GRO beta/MIP‑2/CINC‑3 in ELISAs and Western blots. In sandwich ELISAs, less than 0.5% cross-reactivity with recombinant rat (rr) CXCL3 and less than 0.1% cross-reactivity with rrCXCL1, rrCINC-2 alpha, recombinant mouse (rm) CXCL9 or rmCXCL2 is observed. In Western blots, less than 3% cross-reactivity with recombinant human (rh) CXCL8, recombinant porcine CXCL8, or rrCINC-2 alpha and no cross‑reactivity with rrCXCL1, 3, rmCXCL1, 2, 6, 9, 10, 12, rhCXLC1, 2, 3, 5, 6, 7, 9,10, 11, 12 or 13 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Rat CXCL2/GRO beta/MIP-2/CINC-3 Antibody
Chemotaxis Induced by CXCL2/CINC‑3 and Neutral-ization by Rat CXCL2/ CINC‑3 Antibody.
Recombinant Rat CXCL2/CINC-3 (Catalog # 525-C3) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL2/ CINC-3 (0.1 µg/mL) is neutral-ized (green line) by increasing concentrations of Mouse Anti-Rat CXCL2/CINC-3 Monoclonal Antibody (Catalog # MAB525). The ND50 is typically 0.4-2.0 µg/mL.Applications for Rat CXCL2/GRO beta/MIP-2/CINC-3 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Rat CXCL2/GRO beta/MIP-2/CINC-3 (Catalog # 525-C3)
Sample: Recombinant Rat CXCL2/GRO beta/MIP-2/CINC-3 (Catalog # 525-C3)
Neutralization
Measured by its ability to neutralize CXCL2/GRO beta/MIP-2/CINC-3-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.4-2.0 µg/mL in the presence of 0.1 µg/mL Recombinant Rat CXCL2/GRO beta/MIP-2/CINC-3.
Rat CXCL2/CINC-3 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL2/GRO beta/MIP-2/CINC-3
Cytokine-induced neutrophil chemoattractant 3 (CINC-3) has been found to be expressed by cytokine-stimulated rat alveolar macrophages and fibroblasts. Based on its protein and DNA sequences, CINC-3 (also known as rat MIP-2, GRO beta and CXCL2) is a member of the alpha (CXC) subfamily of chemokines.
CINC-3 cDNA encodes a 100 amino acid (aa) residue precursor protein with a 31 aa signal peptide that is cleaved to yield a 69 aa mature secreted protein. The protein sequence of rat CINC-3 shares approximately 88% identity with murine MIP-2. Characteristic of ELR containing CXC chemokines, CINC-3 is known to be a potent chemotactic factor for rat neutrophils in vitro and in vivo. On the basis of cross-desensitization results of the various CINC proteins, it has been postulated that rat neutrophils have at least two classes of CINC receptors: a class of CINC-3 specific receptors as well as a second common receptor shared by all CINCs.
References
- Nakagawa, H. et al. (1994) Biochem. J. 301:545.
- Huang, S. et al. (1992) Am. J. Pathol. 141:981.
Alternate Names
CINC-3, CINC3, GRO beta, MGSA-beta, MIP-2
Gene Symbol
CXCL2
UniProt
Additional CXCL2/GRO beta/MIP-2/CINC-3 Products
Product Documents for Rat CXCL2/GRO beta/MIP-2/CINC-3 Antibody
Product Specific Notices for Rat CXCL2/GRO beta/MIP-2/CINC-3 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...