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Rat CXCL7/Thymus Chemokine-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF1116

R&D Systems, part of Bio-Techne
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AF1116
AF1116-SP

Key Product Details

Species Reactivity

Rat

Applications

Immunohistochemistry, Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

E. coli-derived recombinant rat CXCL7/Thymus Chemokine-1
Ile46-Ile107
Accession # NP_714943

Specificity

Detects CXCL7/Thymus Chemokine‑1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse CXCL7 is observed and less than 1% cross-reactivity with other chemokines tested.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Rat CXCL7/Thymus Chemokine-1 Antibody

Chemotaxis Induced by CXCL7/Thymus Chemokine-1 and Neutralization by Rat CXCL7/Thymus Chemokine-1 Antibody.

Chemotaxis Induced by CXCL7/Thymus Chemokine-1 and Neutralization by Rat CXCL7/Thymus Chemokine-1 Antibody.

Recombinant Rat CXCL7/Thymus Chemokine-1 (Catalog # 1116-CK) chemo-attracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL7/Thymus Chemokine-1 (0.04 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CXCL7/Thymus Chemokine-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1116). The ND50 is typically 0.8-4.0 µg/mL.

Applications for Rat CXCL7/Thymus Chemokine-1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat thymus

Western Blot

0.1 µg/mL
Sample: Recombinant Rat CXCL7/Thymus Chemokine-1 (Catalog # 1116-CK)

Neutralization

Measured by its ability to neutralize CXCL7/Thymus Chemokine-1-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.8-4.0 µg/mL in the presence of 0.04 µg/mL Recombinant Rat CXCL7/Thymus Chemokine-1.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL7/Thymus Chemokine-1

CXCL7 is a member of the alpha (CXC) subfamily of chemokines. Rat CXCL7 cDNA encodes a 111 amino acid residue precursor protein. Rat CXCL7 shares 72% amino acid sequence identity with mouse CXCL7 (accession number AAG36786). The recombinant rat CXCL7 produced by R&D Systems has been shown to chemoattract human CXCR2 transfected mouse BaF/3 cells, but not human CXCR1 transfected mouse BaF/3 cells.




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Alternate Names

Thymus Chemokine1

Entrez Gene IDs

57349 (Mouse); 246358 (Rat)

Gene Symbol

PPBP

UniProt

Additional CXCL7/Thymus Chemokine-1 Products

Product Documents for Rat CXCL7/Thymus Chemokine-1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Rat CXCL7/Thymus Chemokine-1 Antibody

For research use only

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