Rat EGF Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3214

R&D Systems, part of Bio-Techne
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MAB3214
MAB3214-SP
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Key Product Details

Species Reactivity

Rat

Applications

Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 420610

View all EGF Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rat EGF
Asn974-Arg1026
Accession # P07522

Specificity

Detects rat EGF in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) Epigen, rmEpiregulin, recombinant human (rh) HB-EGF, rhEGF, rmEGF, or rhHRG is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Rat EGF Antibody

Cell Proliferation Induced by EGF and Neutralization by Rat EGF Antibody.

Cell Proliferation Induced by EGF and Neutralization by Rat EGF Antibody.

Recombinant Rat EGF (Catalog # 3214-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat EGF (75 pg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat EGF Monoclonal Antibody (Catalog # MAB3214). The ND50 is typically 0.75-3.75 µg/mL.

Applications for Rat EGF Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Rat EGF (Catalog # 3214-EG)

Neutralization

Measured by its ability to neutralize EGF-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 0.75-3.75 µg/mL in the presence of 75 pg/mL Recombinant Rat EGF.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGF

Epidermal growth factor (EGF) is the founding member of the EGF family that also includes TGF-alpha, amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparin‑binding EGF-like growth factor (HB-EGF), epigen, and the neuregulins (NRG)-1 through -6 (1). Members of the EGF family share a structural motif, the EGF‑like domain, which is characterized by three intramolecular disulfide bonds that are formed by six similarly spaced conserved cysteine residues (2). All EGF family members are synthesized as type I transmembrane precursor proteins that may contain several EGF domains in the extracellular region. The mature proteins are released from the cell surface by regulated proteolysis (1). The 1133 amino acid (aa) rat EGF precursor contains nine EGF domains and nine LDLR class B repeats. The mature protein consists of 53 aa and is generated by proteolytic excision of the EGF domain proximal to the transmembrane region (3). Mature rat EGF shares 70% and 77% aa sequence identity with mature human and mouse EGF, respectively. EGF is present in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid (4). Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members (5). These receptors undergo a complex pattern of ligand induced homo- or hetero-dimerization to transduce EGF family signals (6, 7). EGF binds ErbB1 and depending on the context, induces the formation of homodimers or heterodimers containing ErbB2. Dimerization results in autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules (5, 8). Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.

References

  1. Harris, R.C. et al. (2003) Exp. Cell Res. 284:2.
  2. Carpenter, G. and Cohen, S. (1990) J. Biol. Chem. 265:7709.
  3. Saggi, S.J. et al. (1992) DNA Cell Biol. 11:481.
  4. Carpenter, G. and Zendegui, J.G. (1986) Exp. Cell Res. 164:1.
  5. Jorissen, R.N. et al. (2003) Exp. Cell Res. 284:31.
  6. Gamett, D.C. et al. (1997) J. Biol. Chem. 272:12052.
  7. Qian, X. et al. (1994) Proc. Natl. Acad. Sci. 91:1500.
  8. Qian, X. et al. (1999) J. Biol. Chem. 274:574.

Long Name

Epidermal Growth Factor

Alternate Names

HOMG4, URG, Urogastrone

Entrez Gene IDs

1950 (Human); 13645 (Mouse); 25313 (Rat)

Gene Symbol

EGF

UniProt

P07522

Additional EGF Products

Product Documents for Rat EGF Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Rat EGF Antibody

For research use only

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