Rat EphB1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5422

R&D Systems, part of Bio-Techne
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MAB5422
MAB5422-SP
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Key Product Details

Species Reactivity

Rat

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 917118

View all EphB1 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat EphB1
Met1-Ser538
Accession # P09759

Specificity

Detects rat EphB1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Rat EphB1 Antibody

EphB1 antibody in Rat Brain by Immunohistochemistry (IHC-Fr).

EphB1 in Rat Brain.

EphB1 was detected in perfusion fixed frozen sections of rat brain (dorsal root ganglia) using Mouse Anti-Rat EphB1 Monoclonal Antibody (Catalog # MAB5422) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in small-sized neurons. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Rat EphB1 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of rat brain (dorsal root ganglia)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EphB1

EphB1, also known as Elk, Cek6, Net, and Hek6, is a type I transmembrane receptor tyrosine kinase with two extracellular fibronectin type III domains. EphB1 binds Ephrin B1, B2, and B3 and plays an important role in nervous system development and axonal migration. Within the extracellular domain, rat EphB1 shares 99% amino acid sequence identity with human and mouse EphB1.

Long Name

Eph Receptor B1

Alternate Names

Cek6, Elk, Hek6, Net

Entrez Gene IDs

2047 (Human); 270190 (Mouse); 24338 (Rat)

Gene Symbol

EPHB1

UniProt

P09759

Additional EphB1 Products

Product Documents for Rat EphB1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Rat EphB1 Antibody

For research use only

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