Rat EphB1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5422
Key Product Details
Species Reactivity
Validated:
Rat
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 917118
Product Summary for Rat EphB1 Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat EphB1
Met1-Ser538
Accession # P09759
Met1-Ser538
Accession # P09759
Specificity
Detects rat EphB1 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Rat EphB1 Antibody
EphB1 in Rat Brain.
EphB1 was detected in perfusion fixed frozen sections of rat brain (dorsal root ganglia) using Mouse Anti-Rat EphB1 Monoclonal Antibody (Catalog # MAB5422) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in small-sized neurons. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Applications for Rat EphB1 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed frozen sections of rat brain (dorsal root ganglia)
Sample: Perfusion fixed frozen sections of rat brain (dorsal root ganglia)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EphB1
Long Name
Eph Receptor B1
Alternate Names
Cek6, Elk, Hek6, Net
Gene Symbol
EPHB1
UniProt
Additional EphB1 Products
Product Documents for Rat EphB1 Antibody
Product Specific Notices for Rat EphB1 Antibody
For research use only
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