Rat Growth Hormone Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1566
Key Product Details
Species Reactivity
Validated:
Rat
Cited:
Mouse, Rat, Porcine, Lizard - Gecko
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant rat Growth Hormone
Leu27-Phe216
Accession # P01244
Leu27-Phe216
Accession # P01244
Specificity
Detects rat Growth Hormone in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Growth Hormone is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Rat Growth Hormone Antibody
Growth Hormone in Rat Pituitary.
Growth Hormone was detected in perfusion fixed frozen sections of rat pituitary using Goat Anti-Rat Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1566) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Rat Growth Hormone Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of rat embryo (15 d.p.c.) and perfusion fixed frozen sections of rat pituitary
Sample: Immersion fixed paraffin-embedded sections of rat embryo (15 d.p.c.) and perfusion fixed frozen sections of rat pituitary
Western Blot
0.1 µg/mL
Sample: Rat Growth Hormone
Sample: Rat Growth Hormone
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Growth Hormone
Alternate Names
GH-N, GH1, GHB5, GHN, IGHD1A, IGHD2, Somatotropin
Entrez Gene IDs
2688 (Human)
Gene Symbol
GH1
UniProt
Additional Growth Hormone Products
Product Documents for Rat Growth Hormone Antibody
Product Specific Notices for Rat Growth Hormone Antibody
For research use only
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