Rat IFN-beta Antibody
R&D Systems, part of Bio-Techne | Catalog # 33400-1
Key Product Details
Species Reactivity
Validated:
Rat
Cited:
Rat
Applications
Validated:
Neutralization
Cited:
Neutralization
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant rat IFN-beta
Specificity
Neutralizes both natural and recombinant rat interferon beta. Cross-reactivity with human interferon beta has not been tested.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Applications for Rat IFN-beta Antibody
Application
Recommended Usage
Neutralization
One neutralization unit is the amount of antiserum required to neutralize one unit of recombinant rat interferon beta (rat IFN-beta) to a 50% endpoint (one unit corresponds to approximately 15 pg of purified rat IFN-beta). Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol. 37, 755-758; Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assay for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. As there is no international rat IFN-beta standard,the units are determined with respect to the international reference standard for mouse interferon beta (mouse IFN-beta) provided by the National Institutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23]. This material is prepared specifically for effective neutralization of rat IFN-beta.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Dissolve contents of the vial by injection of 0.5 ml sterile distilled water.
Formulation
0.5 ml Phosphate-buffered saline (PBS) + 125 mM trehalose prior to lyophilization.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Upon receipt, this product is stable at 4°C until the expiration date listed above. After reconstitution, the contents can be safely stored at 4°C for one month or at -20°C for one year for retention of full activity.When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Add 0.02% sodium azide to prevent bacterial growth.
Background: IFN-beta
References
- Ruuls, S.R. et al. (1996) J. Immunol. 157: 5721;
- Zou, L.P. et al. 1999. Neuroscience Res. 56: 123
Long Name
Interferon beta
Alternate Names
IFB, IFF, IFNB, IFNB1, IFNbeta
Gene Symbol
IFNB1
Additional IFN-beta Products
Product Documents for Rat IFN-beta Antibody
Product Specific Notices for Rat IFN-beta Antibody
For research use only
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