Rat M-CSF Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5364
Key Product Details
Species Reactivity
Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 427406
Product Specifications
Immunogen
E. coli-derived recombinant rat M‑CSF
Glu33-Glu261
Accession # Q8JZQ0
Glu33-Glu261
Accession # Q8JZQ0
Specificity
Detects rat M-CSF in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse M‑CSF and recombinant human M‑CSF is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Rat M-CSF Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Rat M-CSF
Sample: Recombinant Rat M-CSF
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: M-CSF
M-CSF, also known as CSF-1, is a four-alpha −helical-bundle cytokine that is essential for survival, proliferation and differentiation of macrophages and osteoclast progenitors. M-CSF is produced by fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells. Full-length rat M-CSF transcripts encode a 534 amino acid (aa) type I transmembrane mature protein. In humans this encodes a 70 kDa protein that forms covalent homodimers. Human 22 kDa, 43 kDa and proteoglycan-modified 200 kDa secreted forms and 34 kDa transmembrane forms may also occur in rats. Mature rat M-CSF shares 89% and 74% aa identity with mouse and human M-CSF, respectively, within the first 229 aa.
Long Name
Macrophage Colony Stimulating Factor
Alternate Names
CSF-1, CSF1, Lanimostim, MCSF
Gene Symbol
CSF1
UniProt
Additional M-CSF Products
Product Documents for Rat M-CSF Antibody
Product Specific Notices for Rat M-CSF Antibody
For research use only
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