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Rat MAG/Siglec-4a Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF538

R&D Systems, part of Bio-Techne
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BAF538

Key Product Details

Species Reactivity

Rat

Applications

ELISA Detection (Matched Antibody Pair), Immunohistochemistry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat MAG/Siglec‑4a
Gly20-Pro516
Accession # P07722

Specificity

Detects rat MAG/Siglec‑4a in ELISAs and Western blots. In sandwich immunoasays, less than 0.2% cross-reactivity with recombinant mouse Nogo R is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Rat MAG/Siglec-4a Biotinylated Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat brain (cerebellum)

Western Blot

0.1 µg/mL
Sample: Recombinant Rat MAG/Siglec-4a Fc Chimera (Catalog # 538-MG)

Rat MAG/Siglec-4a Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Rat MAG/Siglec-4a Antibody (Catalog # MAB5381)
  • Standard: Recombinant Rat MAG/Siglec-4a Fc Chimera Protein, CF (Catalog # 538-MG)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MAG/Siglec-4a

MAG (Myelin-Associated Glycoprotein), a type I transmembrane glycoprotein containing five Ig-like domains in its extracellular domain is an adhesion molecule belonging to the immunoglobin superfamily. Within this superfamily, MAG, CD22, CD33, Schwann cell myelin protein, and sialoadhesin which bind specifically to cell‑surface glycan containing sialic acid residues define the I-type sialyl lectin subgroup, also called the sialoadhesin family. Sialoadhesins mediate diverse biological processes through recognition of specific sialyted glycans on cell surface. MAG is expressed on myelinating oligodenrocytes and Schwann cells, and preferentially recognizes alpha2, 3-linked sialic acid on O-linked glycans and gangliosides. MAG exists as two isoforms which differ in the sequence and length of the cytoplasmic tail. The large form (71 kDa) and small form (67 kDa) arise from alternative spliced mRNAs. Although MAG might encounter haematopoietic cells and lymphocytes under pathologic conditions, it would normally be expected to interact with neuronal cells. It has been shown that MAG promotes axonal growth from neonatal DRG neurons and embryonic spinal neurons, but is a potent inhibitor of axonal re-growth from adult DRG and postnatal cerebellar neurons. MAG plays an important role in the interaction between axons and myelin. A soluble form of MAG containing the extracellular domain is released from myelin in large quantities and identified in normal human tissues and in tissues from patients with neurological disorders. This soluble MAG might contribute to the lack of CNS neuron regeneration after injury.

References

  1. Kelm, S. et al. (1994) Current Biology 4:965.
  2. McKerracher, L. et al. (1994) Neuron 13:805.
  3. Tang, S. et al. (1997) Molecular and Cellular Neuroscience 9:333.
  4. Cai, D. et al. (1999) Neuron 22:89.

Long Name

Myelin-associated Glycoprotein

Alternate Names

Siglec-4a, Siglec4a

Entrez Gene IDs

4099 (Human); 17136 (Mouse); 29409 (Rat)

Gene Symbol

MAG

UniProt

Additional MAG/Siglec-4a Products

Product Documents for Rat MAG/Siglec-4a Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Rat MAG/Siglec-4a Biotinylated Antibody

For research use only

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