Rat MMP-8 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3245
Key Product Details
Species Reactivity
Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 400102
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat MMP-8
Leu21-Pro466
Accession # O88766
Leu21-Pro466
Accession # O88766
Specificity
Detects rat MMP-8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant mouse MMP-8 and 50% cross-reactivity with recombinant human MMP-8 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Rat MMP-8 Antibody
Detection of Rat MMP‑8 by Western Blot.
Western blot shows lysates of rat lung tissue and rat spleen tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Rat MMP-8 Monoclonal Antibody (Catalog # MAB3245) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MMP-8 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Rat MMP-8 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Rat lung tissue and Rat spleen tissue
Sample: Rat lung tissue and Rat spleen tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-8
Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-8 (neutrophil collagenase, collagenase 2) is expressed in neutrophils, where it is stored in specific granules. MMP-8 release from the neutrophils is stimulated by various factors such as interleukins 1 and 8, TNF-alpha and GM-CSF. MMP-8 is capable of cleaving types I, II, and III triple-helical collagen, gelatin peptides, fibronectin, proteoglycans, aggrecan, serpins, beta-casein, and peptides such as angiotensin and substance P. In addition to its function in phagocytosis, MMP-8 has a high capacity for infiltrating connective tissue and is implicated in the breakdown of the extracellular matrix in diseases such as rheumatoid arthritis. Structurally, MMP-8 consists of several domains: a pro-domain that is cleaved upon activation, a catalytic domain containing the zinc-binding site, a short hinge region, and a hemopexin-like domain (1). The amino acid sequence of rat MMP-8 shares 91%, 75% and 73% identity with that of mouse, dog and human, respectively.
References
- Tschesche, T. et al. (2004) Handbook of Proteolytic Enzymes (eds. A.J. Barrett et al.) p.480, Academic Press, San Diego.
Long Name
Matrix Metalloproteinase 8
Alternate Names
Collagenase 2, MMP8
Gene Symbol
MMP8
UniProt
Additional MMP-8 Products
Product Documents for Rat MMP-8 Antibody
Product Specific Notices for Rat MMP-8 Antibody
For research use only
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