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Rat PDGF-AA Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF1055

R&D Systems, part of Bio-Techne
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BAF1055

Key Product Details

Species Reactivity

Rat

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

E. coli-derived recombinant rat PDGF‑AA (R&D Systems, Catalog # 1055-AA)
Ser87-Arg196
Accession # AAB59693

Specificity

Detects rat PDGF-AA in Western blots. In this format, approximately 45% cross-reactivity with recombinant rat PDGF-AB is observed, 35% cross-reactivity with recombinant human PDGF-AA is observed, 5% cross-reactivity with recombinant zebrafish PDGF-AA is observed and less than 1% cross-reactivity with recombinant rat PDGF-BB is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Rat PDGF-AA Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Rat PDGF-AA (Catalog # 1055-AA)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF-AA

The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGF-A, -B, -C, and -D) that form four disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and one heterodimer (PDGF-AB) (1, 2). These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero-dimerization of two receptors (PDGF R alpha and R beta). Whereas alpha/ alpha homo-dimerization is induced by PDGF-AA, -BB, -CC, and -AB, alpha/ beta hetero-dimerization is induced by PDGF-AB,-BB, -CC, and -DD, and beta/ beta homo-dimerization is induced only by PDGF-BB, and -DD (1‑4). Both PDGF R alpha and R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that have five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. Ligand-induced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, cell migration and extracellular matrix production. Rat PDGF-A chain cDNA encodes a 204 amino acid (aa) residue precursor protein with a 20 aa signal peptide, a 65 aa propeptide that is removed by proteolysis, and a 119 aa mature protein. By alternative splicing, a short form lacking 8 C-terminal aa residues also exists. The long form contains the 8 aa basic insert which promotes intracellular cell retention and association with cell matrix. PDGF-A is expressed in multiple cell types and tissues. Based on PDGF-A knockout studies, PDGF-A appears to be important for the development of oligodendrocytes, testicular Leydig cells, alveolar smooth muscle cells, hair follicles and intestinal villus (1).

References

  1. Betshotz, C. et al. (2001) BioEssays 23:494.
  2. Ostman, A. and A.H. Heldin (2001) Advances in Cancer Research 80:1.
  3. Gilbertson, D. et al. (2001) J. Biol. Chem. 276:27406.
  4. LaRochells, W.J. et al. (2001) Nature Cell Biol. 3:517.

Long Name

Platelet-derived Growth Factor AA

Alternate Names

PDGFAA

Entrez Gene IDs

5154 (Human); 25266 (Rat)

Gene Symbol

PDGFA

UniProt

Additional PDGF-AA Products

Product Documents for Rat PDGF-AA Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Rat PDGF-AA Biotinylated Antibody

For research use only

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