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Rat Reg3B Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1996

R&D Systems, part of Bio-Techne
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MAB1996
MAB1996-SP

Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 293523

Product Specifications

Immunogen

E. coli-derived recombinant rat Reg3B
Glu27-Gly175
Accession # P25031

Specificity

Detects rat Reg3B in direct ELISAs and Western blots. Does not cross-react with recombinant mouse Reg1A, 1B, 2A, recombinant rat Reg3, or recombinant human Reg4.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Rat Reg3B Antibody

Reg3B antibody in Rat Pancreas by Immunohistochemistry (IHC-Fr).

Reg3B in Rat Pancreas.

Reg3B was detected in perfusion fixed frozen sections of rat pancreas using Mouse Anti-Rat Reg3B Monoclonal Antibody (Catalog # MAB1996) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of exocrine cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Rat Reg3B Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of rat pancreas (inflammatory conditions)

Western Blot

1 µg/mL
Sample: Recombinant Rat Reg3B under non-reducing conditions only
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Reg3B

Reg3B, also named Pancreatitis-Associated Protein (PAP) and peptide 23, belongs to the Regenerating gene family (Reg), within the superfamily of C-type lectins. It has been classified as a member of the type III subclass of the Reg family. Reg3B has anti-apoptotic effects on pancreatic acinar cells and is a novel motor and sensory neuron survival factor.

Long Name

Regenerating Islet-derived IIIb

Alternate Names

PAP1

Entrez Gene IDs

18489 (Mouse); 24618 (Rat)

Gene Symbol

REG3B

UniProt

Additional Reg3B Products

Product Documents for Rat Reg3B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Rat Reg3B Antibody

For research use only

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