Rat Synaptotagmin-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB43641
Key Product Details
Species Reactivity
Validated:
Rat
Cited:
Rat
Applications
Validated:
Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # ASV30
Product Specifications
Immunogen
Rat brain synaptic plasma membranes
Specificity
Detects rat Synaptotagmin‑1 in Western blots. Detects Synaptotagmin-1 from rat, mouse, human, chicken, Xenopus, and fish.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Rat Synaptotagmin-1 Antibody
Synaptotagmin-1 in Rat Spinal Cord.
Synaptotagmin-1 was detected in perfusion fixed frozen sections of rat spinal cord using 8 µg/mL Mouse Anti-Rat Synaptotagmin-1 Monoclonal Antibody (Catalog # MAB43641) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Applications for Rat Synaptotagmin-1 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed frozen sections of rat spinal cord
Sample: Perfusion fixed frozen sections of rat spinal cord
Immunoprecipitation
Matthew, W.D. et al. (1981) J. Cell Biol. 91:257.
Western Blot
Matthew, W.D. et al. (1981) J. Cell Biol. 91:257. This application was not tested by R&D Systems.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Synaptotagmin-1
The Synaptotagmins are a family of integral membrane proteins of synaptic vesicles. Rat Synaptotagmin-1 (Accession # P21707) is a 57 kDa, 421 aa glycoprotein containing two C2 domains related to protein kinase C, and sites for palmitoylation and binding of acidic phospholipids, calcium and calmodulin. Synaptotagmin-1 participates in the process of vesicular trafficking and exocytosis by inducing local Ca2+-dependent buckling of the plasma membrane. Rat Synaptotagmin-1 shares 98% aa identity or greater with mouse, human and bovine Synaptotagmin-1.
Alternate Names
P65, SVP65, Synaptotagmin1, SYT1
Gene Symbol
SYT1
Additional Synaptotagmin-1 Products
Product Documents for Rat Synaptotagmin-1 Antibody
Product Specific Notices for Rat Synaptotagmin-1 Antibody
For research use only
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