Rat TIMP-1 Antibody

Name: Rat TIMP-1 Antibody
Brand: R&D Systems
SKU: MAB5802

R&D Systems, part of Bio-Techne | Catalog # MAB5802

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Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Rat

Applications

ELISA Capture (Matched Antibody Pair)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 145701

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Product Summary for Rat TIMP-1 Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat TIMP-1
Cys24-Ala217
Accession # P30120

Specificity

Detects recombinant rat TIMP-1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for Rat TIMP-1 Antibody

Application

Recommended Usage

Rat TIMP-1 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Rat TIMP-1 Biotinylated Antibody (Catalog # BAF580)
Standard: Recombinant Rat TIMP-1 Protein, CF (Catalog # 580-RT)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIMP-1

Tissue Inhibitor of Metalloproteinases-1, (TIMP-1) belongs to a family of proteins that regulate the activation and proteolytic activity of the matrix metalloproteinases (MMPs). TIMP-1 is a glycoprotein produced by a wide range of cell types. It inhibits active MMP-mediated proteolysis by forming an N-terminal, non-covalent binary complex with the MMP active site. TIMP-1 also associates C-terminally with Pro-MMP-9 in a complex that may play a role in regulating activation. Independent of MMPs, TIMP-1 has been shown to play a role in tissue homeostasis.

Long Name

Tissue Inhibitors of Metalloproteinases 1

Alternate Names

TIMP1

Entrez Gene IDs

7076 (Human); 21857 (Mouse); 116510 (Rat)

Gene Symbol