Rat TIMP-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB580
Key Product Details
Species Reactivity
Validated:
Rat
Cited:
Rat
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 150911
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat TIMP-1
Cys24-Ala217
Accession # P30120
Cys24-Ala217
Accession # P30120
Specificity
Detects rat TIMP-1 in direct ELISAs and Western blots. Does not cross-react with recombinant mouse TIMP‑1, recombinant human (rh) TIMP-1, rhTIMP-2, rhTIMP-3, or rhTIMP-4.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Rat TIMP-1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Rat TIMP-1 (Catalog # 580-RT)
Sample: Recombinant Rat TIMP-1 (Catalog # 580-RT)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TIMP-1
Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3 and TIMP-4. TIMP-1 is a glycoprotein with a molecular mass of 32 - 34 kDa produced by a wide range of cell types. TIMP-1 inhibits active MMP-mediated proteolysis by forming an N-terminal, non-covalent binary complex with the MMP active site. TIMP-1 also associates C-terminally with pro-MMP-9 in a complex which may play a role in regulating activation. Independent of MMPs, TIMP-1 has been shown to have a role in tissue homeostasis.
Long Name
Tissue Inhibitors of Metalloproteinases 1
Alternate Names
TIMP1
Gene Symbol
TIMP1
UniProt
Additional TIMP-1 Products
Product Documents for Rat TIMP-1 Antibody
Product Specific Notices for Rat TIMP-1 Antibody
For research use only
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