RB1 Antibody (172C1094) - (cleaved) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33240
![Western Blot: RB1 Antibody (172C1094)(cleaved)Azide and BSA Free [NBP2-33240]](https://resources.bio-techne.com/images/products/RB1-Antibody-172C1094-cleaved-Azide-Free-Western-Blot-NBP2-33240-img0002.jpg "Western Blot: RB1 Antibody (172C1094)(cleaved)Azide and BSA Free [NBP2-33240]")
![Western Blot: RB1 Antibody (172C1094)(cleaved)Azide and BSA Free [NBP2-33240]](https://resources.bio-techne.com/images/products/RB1-Antibody-172C1094-cleaved-Azide-Free-Western-Blot-NBP2-33240-img0003.jpg "Western Blot: RB1 Antibody (172C1094)(cleaved)Azide and BSA Free [NBP2-33240]")
Key Product Details
Validated by
Knockout/Knockdown, Biological Validation
Species Reactivity
Validated:
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Knockdown Validated, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 172C1094
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Summary for RB1 Antibody (172C1094) - (cleaved) - Azide and BSA Free
Immunogen
A synthetic peptide corresponding to a region of the caspase-3-induced cleavage site of human Rb was used as immunogen.
Specificity
The antibody recognizes cleaved Rb (DRb-p70). The antibody does not recognize full-length Rb. In camptothecin treated HL60, an ~68 kDa band is observed. DRb-p70 exists in vivo as an N-terminally truncated form of Rb (Liu et al.). DRb-p70 is thought to be the product of alternative translation and is expressed in primary myeloid cells in fetal liver, bone marrow and spleen. DRb-p70 is also specifically expressed in tumor-associated macrophages (TAMs). TAMs are an important targets of cancer therapy. DRb-p70 is a marker of TAMs, and maybe useful for identifying tumors with macrophage infiltration (Liu et al, 2005).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for RB1 Antibody (172C1094) - (cleaved) - Azide and BSA Free
Western Blot: RB1 Antibody (172C1094)(cleaved)Azide and BSA Free [NBP2-33240]
Western Blot: RB1 Antibody (172C1094) - (cleaved) - Azide Free [NBP2-33240] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and RB-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Mouse Anti-Human RB-1 Monoclonal Antibody (Catalog # NB100-56598) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for RB-1 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions. Image using the standard format of this product.![Immunohistochemistry: RB1 Antibody (172C1094) - (cleaved) - Azide and BSA Free [NBP2-33240]](https://resources.bio-techne.com/images/products/RB1-Antibody-172C1094-cleaved-Azide-Free-Immunohistochemistry-NBP2-33240-img0001.jpg "Immunohistochemistry: RB1 Antibody (172C1094) - (cleaved) - Azide and BSA Free [NBP2-33240]")
Immunohistochemistry: RB1 Antibody (172C1094) - (cleaved) - Azide and BSA Free [NBP2-33240]
Immunohistochemistry: RB1 Antibody (172C1094) - (cleaved) - Azide Free [NBP2-33240] - analysis in sections of primary human breast tumors. Image courtesy of Dr. Macleod, The Ben May Institute for Cancer Research, The University of Chicago.Applications for RB1 Antibody (172C1094) - (cleaved) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Western Blot
2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RB1
Long Name
Retinoblastoma-associated protein
Alternate Names
OSRC, p105-Rb, pp110
Gene Symbol
RB1
Additional RB1 Products
Product Documents for RB1 Antibody (172C1094) - (cleaved) - Azide and BSA Free
Product Specific Notices for RB1 Antibody (172C1094) - (cleaved) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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