RB1 Antibody (rRB1/8060)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23828
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 kappa Clone # rRB1/8060
Concentration
0.2 mg/ml
Product Summary for RB1 Antibody (rRB1/8060)
Immunogen
Recombinant human RB1 protein fragment of 283 amino acid residues.
Localization
Nucleus.
Specificity
Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. Its epitope is localized between aa 703-772. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Store at 2 to 8C. Non-hazardous. No MSDS required.
Applications for RB1 Antibody (rRB1/8060)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2ug/ml
Western Blot
1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: RB1
Long Name
Retinoblastoma-associated protein
Alternate Names
OSRC, p105-Rb, pp110
Gene Symbol
RB1
Additional RB1 Products
Product Documents for RB1 Antibody (rRB1/8060)
Product Specific Notices for RB1 Antibody (rRB1/8060)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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