RB1 Antibody (SPM353) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54477
![Immunohistochemistry-Paraffin: RB1 Antibody (SPM353) - Azide and BSA Free [NBP2-54477]](https://resources.bio-techne.com/images/products/RB1-Antibody-SPM353-Immunohistochemistry-Paraffin-NBP2-54477-img0001.jpg "Immunohistochemistry-Paraffin: RB1 Antibody (SPM353) - Azide and BSA Free [NBP2-54477]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM353
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for RB1 Antibody (SPM353) - Azide and BSA Free
Immunogen
Recombinant human RB1 protein fragment of 283 amino acid residues. (Uniprot: P06400)
Epitope
aa 703-772
Localization
Nuclear
Specificity
Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. Its epitope is localized between aa 703-772. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
105 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53399).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for RB1 Antibody (SPM353) - Azide and BSA Free
Immunohistochemistry-Paraffin: RB1 Antibody (SPM353) - Azide and BSA Free [NBP2-54477]
Immunohistochemistry-Paraffin: RB1 Antibody (SPM353) [NBP2-54477] - Formalin-fixed, paraffin-embedded human Colon stained with Retinoblastoma (Rb1) Monoclonal Antibody (SPM353).Applications for RB1 Antibody (SPM353) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1 - 2 ug/ml
Immunoprecipitation
0.5 - 1 ug
Western Blot
0.5 - 1 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: RB1
Long Name
Retinoblastoma-associated protein
Alternate Names
OSRC, p105-Rb, pp110
Gene Symbol
RB1
Additional RB1 Products
Product Documents for RB1 Antibody (SPM353) - Azide and BSA Free
Product Specific Notices for RB1 Antibody (SPM353) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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