RBBP4/RbAp48 Antibody (3E9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26350
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3E9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for RBBP4/RbAp48 Antibody (3E9)
Immunogen
A synthesized peptide derived from Human RBBP4/RbAp48 [UniProt Q09028]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RBBP4/RbAp48 Antibody (3E9)
Immunohistochemistry: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] -
Immunohistochemistry: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Image of RBBP4/RbAp48 Antibody (3E9) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] -
Immunohistochemistry: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Image of RBBP4/RbAp48 Antibody (3E9) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] -
Western Blot: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Positive Western Blot detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, HEK293 whole cell lysate, L02 whole cell lysate, Jurkat whole cell lysate, SH-SY5Y whole cell lysate, Mouse Brain whole cell lysate, Rat Brain cell lysate.All lanes: RBBP4/RbAp48 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 48, 48, 47, 44 kDa
Observed band size: 53, 40 kDa
Applications for RBBP4/RbAp48 Antibody (3E9)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:20-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: RBBP4
Long Name
Retinoblastoma Binding Protein 4
Alternate Names
CAF-1 p48, CAF-1C, NURF55, RBAP48
Gene Symbol
RBBP4
Additional RBBP4 Products
Product Documents for RBBP4/RbAp48 Antibody (3E9)
Product Specific Notices for RBBP4/RbAp48 Antibody (3E9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...