RBFOX3/NeuN Antibody (1B7)

Flow Cytometry of SH-SY5Y Cells Stained with Allophycocyanin Conjugated RBFOX3/NeuN Antibody

Using the Allophycocyanin direct conjugate An intracellular stain was performed on SH-SY5Y cells with RBFOXP3/NeuN (1B7) antibody NBP1-92693APC (blue) and a matched isotype control NB600-986APC (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.

Flow Cytometry of SH-SY5Y Cells Stained with Alexa Fluor 647 Conjugated RBFOX3/NeuN Antibody

An intracellular stain was performed on SH-SY5Y cells with RBFOXP3/NeuN (1B7) antibody NBP1-92693AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Immunocytochemistry/Immunofluorescence Imaging of RBFOX3/NeuN in Dissociated Spinal Cord Nuclei

Dissociated spinal cord nuclei immunostained with anti-NeuN antibody and Alexa 488 secondary. DAPI (Blue) to stain nuclei. This image was submitted by customer review.

Flow Cytometry of U87-MG Cells Stained with Alexa Fluor 700 Conjugated RBFOX3/NeuN Antibody

An intracellular stain was performed on U87-MG cells with RBFOXP3/NeuN (1B7) antibody NBP1-92693AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.

Immunocytochemistry/Immunofluorescence Staining of RBFOX3/NeuN in Rat Brain Neural Cultures

Rat brain neural cultures stained with NBP1-92693 (red), a chicken polyclonal antibody to GFAP (NBP1-05198, green) and DAPI (blue). NBP1-92693 reveals strong nuclear and distal cytoplasmic staining for RBFOX/Neun and the complete absence of staining of astrocytes, which are staining with the GFAP antibody, and other kinds of non-neuronal cells. This RBFOX/Neun (1B7) antibody is therefore an excellent marker of neuronal cells.

Immunohistochemical Analysis of RBFOX3/NeuN in Frozen Mouse Spinal Cord

IHC-Fr analysis of a 4% PFA fixed mouse spinal cord tissue section using RBFOX3/NeuN antibody (clone 1B7) at 1:500 dilution with overnight 4C incubation in a dilution buffer which contained BSA 1% and 0.2% TritonX100 in PBS. The signal was detected using Alexa Fluor 594 conjugated goat anti-mouse IgG (H+L) secondary antibody. The antibody generated a specific cytoplasmic-nuclear signal and the staining was more intense in the nuclei of the neurons. This image was submitted as a review via a verified end user of this product.

Flow Cytometry of SH-SY5Y Cells Stained with Phycoerythrin Conjugated RBFOX3/NeuN Antibody

Analysis of PE conjugate of NBP1-92693. An intracellular stain was performed on SH-SY5Y cells with RBFOX3 (1B7) antibody NBP1-92693PE (blue) and a matched isotype control NB600-986PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Flow Cytometry of SH-SY5Y Cells Stained with PerCP Conjugated RBFOX3/NeuN Antibody

Using the PerCP direct conjugate An intracellular stain was performed on SH-SY5Y cells with RBFOXP3/NeuN (1B7) antibody NBP1-92693PCP (blue) and a matched isotype control NB600-986PCP (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Peridinin-Chlorophyll-protein.

Flow Cytometry of SH-SY5Y Cells Stained with Alexa Fluor 700 Conjugated RBFOX3/NeuN Antibody

An intracellular stain was performed on SH-SY5Y cells with RBFOXP3/NeuN (1B7) antibody NBP1-92693AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700. Image from the Alexa Fluor 700 version of this antibody.

Flow Cytometry of A549 Cells Stained with FITC Conjugated RBFOX3/NeuN Antibody

An intracellular stain was performed on A549 cells with RBFOXP3/NeuN (1B7) antibody NBP1-92693F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.

Immunocytochemistry/ Immunofluorescence: RBFOX3/NeuN Antibody (1B7) [NBP1-92693] -

Immunocytochemistry/ Immunofluorescence: RBFOX3/NeuN Antibody (1B7) [NBP1-92693] - NF kappaB localizes to both neuronal & glial nuclei in superior colliculus. Representative confocal images of NF kappaB in the superior colliculus (SC) of vehicle- & HE3286-treated rats with all nuclei indicated (DAPI). Sections were also labeled for astrocytes (A, GFAP), microglia (B, Iba1), or neurons using antibodies against either NeuN (C) or phosphorylated neurofilament heavy (pNFH; D). Representative nuclei from each cell class demonstrated NF kappaB localization (arrows). Insets (solid white, B) show region contained within dashed box with red channel removed to better visualize nuclear localization of NF kappaB. Scale: 10 μm. Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/fnins.2017.00045/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.