RBFOX3/NeuN Antibody (NEUN/7167) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20797
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # NEUN/7167
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa1-200) of human RBFOX3/NeuN protein (exact sequence is proprietary)
Localization
Nucleus. Cytoplasm.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
Positive Controls: Human brain. HeLa cells.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for RBFOX3/NeuN Antibody (NEUN/7167) - Azide and BSA Free
RBFOX3/NeuN Antibody (NEUN/7167)
Formalin-fixed, paraffin-embedded human stomach stained with RBFOX3/NeuN antibody (NEUN/7167). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
RBFOX3/NeuN Antibody (NEUN/7167)
Formalin-fixed, paraffin-embedded human placenta stained with RBFOX3/NeuN antibody (NEUN/7167). Inset: PBS instead of primary antibody; secondary only negative control.
RBFOX3/NeuN Antibody (NEUN/7167)
Formalin-fixed, paraffin-embedded human brain stained with RBFOX3/NeuN antibody (NEUN/7167). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for RBFOX3/NeuN Antibody (NEUN/7167) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: RBFOX3/NeuN
Rbfox proteins participate in regulation of alternative splicing between family members as well as in autoregulation. While the breadth of brain and muscle specific targets for alternative splicing is well established for Rbfox proteins, those specific to Rbfox3/NeuN along with its alternative splicing mechanism are less understood. Rbfox3 has been shown to regulate neuronal differentiation by alternative splicing of Numb pre-mRNA and has a role in adult neurogenesis. Dysfunctional Rbfox3/NeuN has been associated with various neurological disorders such as neurodevelopmental delay, autism spectrum disorder, Benign rolandic epilepsy (BRE), and cognitive impairments (1,2).
References
1. Duan W, Zhang YP, Hou Z, Huang C, Zhu H, Zhang CQ, Yin Q. (2016) Novel Insights into NeuN: from Neuronal Marker to Splicing Regulator. Mol Neurobiol. 53(3):1637-1647. PMID: 25680637.
2. Su CH, D D, Tarn WY. (2018) Alternative Splicing in Neurogenesis and Brain Development. Front Mol Biosci. 5:12. PMID: 29484299
Long Name
RNA binding fox-1 homolog 3
Alternate Names
FOX-3, FOX3, HRNBP3, NEUN
Gene Symbol
RBFOX3
Additional RBFOX3/NeuN Products
Product Documents for RBFOX3/NeuN Antibody (NEUN/7167) - Azide and BSA Free
Product Specific Notices for RBFOX3/NeuN Antibody (NEUN/7167) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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