RBFOX3/NeuN Antibody (PD01-45)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32644
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # PD01-45
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within human RBFOX3/NeuN aa 20-60. (Uniprot: A6NFN3)
Localization
Cytoplasm, Nucleus
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for RBFOX3/NeuN Antibody (PD01-45)
Immunohistochemistry-Frozen: RBFOX3/NeuN Antibody (PD01-45) [NBP3-32644]
Immunohistochemistry-Frozen: RBFOX3/NeuN Antibody (PD01-45) [NBP3-32644] - Immunofluorescence analysis of frozen mouse hippocampus tissue labeling RBFOX3/NeuN with Mouse anti-RBFOX3/NeuN antibody (NBP3-32644).The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32644, green) at 1/50 dilution overnight at 4℃, washed with PBS. Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).
Western Blot: RBFOX3/NeuN Antibody (PD01-45) [NBP3-32644]
Western Blot: RBFOX3/NeuN Antibody (PD01-45) [NBP3-32644] - Western blot analysis of RBFOX3/NeuN on mouse cerebellum tissue lysates with Mouse anti-RBFOX3/NeuN antibody (NBP3-32644) at 1/1,000 dilution.Lysates/proteins at 20 ug/Lane.
Predicted band size: 34 kDa
Observed band size: 45/50 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32644) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.
Immunohistochemistry: RBFOX3/NeuN Antibody (PD01-45) [NBP3-32644]
Immunohistochemistry: RBFOX3/NeuN Antibody (PD01-45) [NBP3-32644] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-RBFOX3/NeuN antibody (NBP3-32644) at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32644) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Applications for RBFOX3/NeuN Antibody (PD01-45)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50
Immunohistochemistry-Frozen
1:50
Immunohistochemistry-Paraffin
1:1000
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RBFOX3/NeuN
Rbfox proteins participate in regulation of alternative splicing between family members as well as in autoregulation. While the breadth of brain and muscle specific targets for alternative splicing is well established for Rbfox proteins, those specific to Rbfox3/NeuN along with its alternative splicing mechanism are less understood. Rbfox3 has been shown to regulate neuronal differentiation by alternative splicing of Numb pre-mRNA and has a role in adult neurogenesis. Dysfunctional Rbfox3/NeuN has been associated with various neurological disorders such as neurodevelopmental delay, autism spectrum disorder, Benign rolandic epilepsy (BRE), and cognitive impairments (1,2).
References
1. Duan W, Zhang YP, Hou Z, Huang C, Zhu H, Zhang CQ, Yin Q. (2016) Novel Insights into NeuN: from Neuronal Marker to Splicing Regulator. Mol Neurobiol. 53(3):1637-1647. PMID: 25680637.
2. Su CH, D D, Tarn WY. (2018) Alternative Splicing in Neurogenesis and Brain Development. Front Mol Biosci. 5:12. PMID: 29484299
Long Name
RNA binding fox-1 homolog 3
Alternate Names
FOX-3, FOX3, HRNBP3, NEUN
Gene Symbol
RBFOX3
Additional RBFOX3/NeuN Products
Product Documents for RBFOX3/NeuN Antibody (PD01-45)
Product Specific Notices for RBFOX3/NeuN Antibody (PD01-45)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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